谷氨酰胺对重症急性胰腺炎大鼠胰腺细胞凋亡的影响

目的:研究谷氨酰胺(Gln)对重症急性胰腺炎(SAP)大鼠胰腺细胞凋亡的影响.方法:将80只大鼠随机分成Gln组(n=36)、SAP组(n=36)和对照组(n=8).术后12、24和36 h处死.用高效液相色谱仪测定血浆和胰腺组织内Gln浓度变化,取大鼠胰腺组织,采用原位末端标记法检测胰腺细胞凋亡指数.结果:SAP时,血浆和胰腺组织内Gln浓度明显下降,经肠道补充Gln能明显提高血浆和胰腺组织内Gln浓度.应用Gln治疗可使SAP大鼠胰腺细胞凋亡指数上升,术后12 h,SAP组为(6.88±0.52)个/100细胞,Gln组为(13.07±0.62)个/100细胞,SAP组相比,其他时点差异也均有显著性意义(P＜0.01).结论:SAP时,应用Gln具有促进胰腺细胞凋亡的作用.

The effects of glutamine on the apoptosis of pancreatic acinus in rats with SAP

Objective:To investigate the role of glutamine on apoptosis of pancreatic acinus in SAP rats.Methods:Eighty SD rats were randomly allocated to 3 groups :glutamine group(Gln group,n=36),SAP group(n=36) and control group(n=8).Jejunotomy was performed.Gln group received glutamine,and SAP group received saline.All groups were subdivided into 3 groups.Eight normal rats in control group were used as controls.At 12,24 and 36 hours after the induction of SAP,twelve rats in each group were sacrificed.Plasma and pancreatic acinar tussue glutamine concentrations were measured by HPLC.The apoptosis of pancreatic acinar cells was detected by TUNEL method.Results:The apoptotic rate of pancreatic acinar in SAP group was much higher than that in control group(P<0.01),and it was higher in Gln group than that in SAP group(P<0.01).Conclusion:Enteral administration of glutamine can increase the apoptotic rate of pancreatic acinar cells in SAP rats.