Nbn 基因对小鼠海马星形胶质细胞发育的影响

目的 观察Nbn 基因神经特异性敲除(Nbn-CNS-del)小鼠海马星形胶质细胞发育的形态学变化，探讨Nbn 基因在小鼠海马星形胶质细胞发育中的作用。方法 采用星形胶质细胞标记物GFAP，应用免疫组织化学技术和体视学方法对生后（P）7、14、21 d 的Nbn-CNS-del小鼠海马进行系统观察和定量分析，以非基因敲除(Nbn-CNS-ctr)的同窝仔鼠为对照组。结果 P7～21 d，与对照组相比，Nbn-CNS-del小鼠海马CA区及齿状回各层GFAP阳性细胞失去正常星形结构，面数密度减小（P＜0.05）。结论 Nbn 基因神经特异性敲除小鼠海马星形胶质细胞发育障碍，Nbn 基因可能在海马神经胶质细胞发育中起重要作用。

Effect of Nbn gene on the development of Hippocampus astrocytes

Objective To investigate the role of Nbn gene on the development of hippocampus astrocytes in Nbn gene neuro-specific deleted (Nbn-CNS-del) mice by studying their morphological changes. Methods GFAP monocloned antigen, immunohistochemistry and stereological methods were used to analyse the morphological changes of hippocampus astrocytes in Nbn-CNS-del and Nbn-CNS-ctr on postnatal day 7, 14 and 21. Results Comparing with Nbn-CNS-ctr, the GFAP positive cells of Nbn-CNS-del mice hippocampus lost their normal structures and the profile numberical densities of GFAP positive cells were decreased（P＜0.05）. Conclusion The development of hippocampus astrocytes in Nbn-CNS-del mice was disordered and Nbn gene might play an important role in the development of hippocampus astrocytes.