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石沙参多糖提取工艺优选及含量测定
引用本文:韩荣春,白增华.石沙参多糖提取工艺优选及含量测定[J].山西中医学院学报,2009,10(1):25-27.
作者姓名:韩荣春  白增华
作者单位:1. 辽宁中医药大学药学院,辽宁,大连,16600
2. 辽宁中医药大学针推学院,辽宁沈阳,110032
摘    要:目的:通过对桔梗科植物石沙参根中多糖成分进行提取工艺研究和含量测定,为石沙参的深入利用和开发提供实验依据和基础资料。方法:以乙醇浓度、浸提次数、浸提时间和浸提温度为考察指标,采用正交设计法对石沙参中水溶性多糖进行提取优化实验;利用紫外检测器对石沙参中的多糖成分进行含量测定和方法学考察。结果:提取温度100℃,提取次数3次,提取时间2h,醇析浓度85%为最佳提取条件;石沙参中水溶性多糖的提取率为8.5%,多糖相对含量为89%。结论:本实验方法简便,实验结果准确,重现性好。

关 键 词:石沙参  水溶性多糖  提取  正交设计  含量

Study on Extraction Technology of Polysaccharide in Adenophora polyantha Nakai
Han Rongchun,Bai Zenghua.Study on Extraction Technology of Polysaccharide in Adenophora polyantha Nakai[J].Journal of Shanxi College of Traditional Chinese Medicine,2009,10(1):25-27.
Authors:Han Rongchun  Bai Zenghua
Institution:1.Medical College;Liaoning University of TCM;Dalian Liaoning 116600;2. College of Acupuncture and Moxibustion;Liaoning Univer-sity of TCM;Shenyang Liaoning 110032
Abstract:Objective:To optimize extraction process and condition of water soluble polysaccharide from the root of Adenophora polyantha Nakai. Methods:The effects of alcohol concentration,extraction time,extraction times and extrac-tion temperature on extraction rate and content of polysaccharide were evaluated by orthogonal design. The content of polysaccharide was detected by ultraviolet detector. Results:The optimum extraction condition of polysaccharide from the root of Adenophora polyantha Nakai was:85% ethanol,3...
Keywords:adenophora polyantha Nakai  water soluble polysaccharide  extraction  orthogonal design  content  
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