HPLC法测定人血清中万古霉素的浓度

目的:建立以高效液相色谱法测定人血清中万古霉素浓度的方法。方法:血清样品以20%偏磷酸溶液沉淀蛋白,离心后取上清液直接进样测定。其中,色谱柱为Hypersil C18,流动相为乙腈-0.0125mol·L-1磷酸二氢钾缓冲液(10∶90),流速为0.8mL·min-1,柱温为25℃,检测波长为236nm。结果:万古霉素血药浓度在2.0～170.0mg·L-1范围内线性关系良好(r=0.9998),最低检测浓度为2.0mg·L-1;低、中、高(2.5、30.8、150.4mg·L-1)3种浓度的平均相对回收率分别为99.8%、101.1%、98.9%,日内及日间RSD均<2.5%(n=5)。结论:本方法简便、准确、快速,适用于人血清万古霉素浓度的监测和药动学研究。

Determination of Vancomycin in Human Serum by HPLC

OBJECTIVE： To establish an HPLC method for the determination of the concentration of vancomycin in human serum. METHODS： Serum samples were centrifuged after serum protein was precipitated by 20% metaphosphoric acid. The separation was performed on Hypersil C18 column with mobile phase consisted of acetonitrile-0.012 5 mol·L-1 potassium dihydrogen phosphate buffer （10 ∶ 90） at flow rate of 0.8 mL·min-1. The detection wavelength was set at 236 nm and column temperature was set at 25 ℃. RESULTS： The average relative recovery rate of vancomycin in low, medium and high concentrations （2.5,30.8,150.4 mg·L-1） were 99.8%, 101.1% and 98.9% respectively. The inter-day and intra-day RSD were less than 2.5%（n=5）. The limit detection of vancomycin in serum was 2.0 mg·L-1. The linear range was within 2.0～170.0 mg·L-1（r=0.999 8）. CONCLUSION： The method is simple, accurate, and rapid for the monitoring and phamacokinetic studies of vancomycin in human serum.