伤寒杆菌聚合酶链反应检测方法

目的 探讨聚合酶链反应快速检出血培养物中的伤寒杆菌。用于伤寒杆菌菌血症的诊断．方法 根据伤寒杆菌鞭毛抗原基因ＤＮＡ核苷序列设计特异性革酸引物,应用聚合酶链反应技术快速检出血培养物中的伤寒杆菌。结果 对伤寒杆菌和７株非伤寒要直菌,同时进行ＰＣＲ增,结果只有伤寒杆菌出现４５８ｂｐ的牧场划性扩增区带,其他７株非伤寒 杆菌均未出现４５８ｂｐ的扩增菌悬液进行扩增,结果表明,在１００个菌细胞时即可扩增出明显的

Rapid detection of typhimurium bacteremia by polymerase chain reaction

AIM To study clinical feasibility of rapid detection of typhimurium bactermia in blood culture by polymerase chain reaction(PCR). METHODS A pair of oligo nucleotide primers were designed by according to H antigen of typhimurium and a PCR system was established to detect typhimurium. RESULTS The 458 bp special strip was found only in the PCR amplification of typhimurium sample,but not in the other seven non typhimurium samples. The sensitivity test indicated that it could detect 100 bacterial cells. And in the simulated blood culture samples of typhimurium,the positive rate came to 80% at 6 hours and 100% at 8 hours of culture time of 18 cases tested by breakaway and PCR,5 cases were proved by breakaway to be masculine;6 cases by PCR to be masculine. As for test hours, PCR (8 h) was distinctly earlier than breakaway(48 h). CONCLUSION The PCR system we established can provide early diagnostic proofs for typhimurium bacteremia.