| SD大鼠胚胎神经干细胞分离、培养及鉴定 |
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| 引用本文： | 冯力,于如同,王婷,董红艳,燕景风,孔令胜. SD大鼠胚胎神经干细胞分离、培养及鉴定[J]. 临床神经外科杂志, 2005, 2(3): 104-106 |
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| 作者姓名： | 冯力 于如同 王婷 董红艳 燕景风 孔令胜 |
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| 作者单位： | 1. 江苏省徐州医学院附属医院神经外科,221002
2. 江苏省徐州医学院神经生物学教研室 |
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| 基金项目： | 江苏省自然科学基金（BK2003025） |
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| 摘 要： | 目的 体外培养并鉴定神经干细胞，为相关实验研究奠定基础。方法 分离SD大鼠胎鼠的间脑，加入神经生长因子EGF和bFGF在神经干细胞条件培养基中克隆培养。用免疫细胞化学方法鉴定分离的神经干细胞。结果 分离培养的细胞具有不断增殖的能力，表达神经巢蛋白（nestin），并能经过诱导分化为神经元和神经胶质细胞。结论 成功建立了神经干细胞的分离培养方法，可用于进一步的实验研究。
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| 关 键 词： | 神经干细胞 细胞培养 胚胎 细胞鉴定 |
| 文章编号： | 1672-7770（2005）03-0104-03 |
| 收稿时间： | 2004-12-13 |
| 修稿时间： | 2005-03-31 |
Isolation,culture and identification of neural stem cells from SD rat embryo |
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| FENG Li , YU Ru-tong , WANG Ting ,et al.. Isolation,culture and identification of neural stem cells from SD rat embryo[J]. Journal of Clinical Neurosurgery, 2005, 2(3): 104-106 |
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| Authors: | FENG Li YU Ru-tong WANG Ting et al. |
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| Affiliation: | FENG Li , YU Ru-tong , WANG Ting , et al. |
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| Abstract: | Objective To cultivate and identify the neural stem cells in vitro for futher related research. Methods Tissues of diencephalon were isolated from a SD rat embryo, then the neural stem cells were cultivated in special culture medium containing bFGF and EGF. The cells were identified by immunocytochemistry. Rusults The cultivated cells have the ability to proliferate continuously . They expressed nestin protein and were differentiated into neurons and glial cells by inducement. Conclusion The method of isolation and cultivation were successfully established and can be used for further research. |
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| Keywords: | neural stem cell cell culture embryo cell identification |
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