RhD（-）非血缘献血者Del的检测分析及检测方法的探讨

本研究旨在分析RhD（-）非血缘献血者中Del的检测，采用血清学方法初筛RhD，用间接抗人球蛋白试验（IAT）确认RhD（-），应用热-乙醚吸收放散法检测Del。结果表明：在38526名健康无偿献血者中，常规血清学方法初筛和IAT试验确认RhD（-）者为106人，热-乙醚吸收放散试验确认Del型者28例，在RhD（-）中的比例为26．41％，Del中各表型的频率分别是Ccee22例，占78．57％，CCee4例，占14．29％，CcEe2例，占7．14％。结论：用热一乙醚吸收放散实验检测Del对合理运用Rh阴性血源具有重要意义，本地区人群的Del表型频率与中国不同区域人群及日本人群Del表型频率有差异。

Analysis on Del Detection of RhD(-)in Unrelated Blood Donors

The study was aimed to analyz Del phenotype of RhD （ - ） unrelated blood donors. RhD （ - ） was initially screened by routine serological test and confirmed by indirect antiglobulin test （IAT）. Del phenotype was detected by hot-ether absorption-elution test. The results indicated that 106 RhD（ - ）samples were confirmed out of 38526 donors, and 28 cases were Del detected by hot-ether absorption-elution test. The incidence of Del in RhD （ - ） samples was 26. 41%, The serological phenotypes of Del were Ccee（78.57% ）,CCee （14.29%）and CcEe （7.14%） respectively. In conclusion, the detection of Del by using hot-ether absorption-elution test is very important for reasonable application of RhD（ - ）blood. There is difference in Del phenotypes of populations in different regions of China and Japan.