Foxp3在神经母细胞瘤LA-N-6细胞的表达及化疗药物对其影响

目的：研究神经母细胞瘤细胞是否表达免疫逃逸相关分子Foxp3及其对化疗药物的敏感性。方法：体外培养神经母细胞瘤细胞株LA-N-6；MTT试验明确化疗药物环磷酰胺（CTX）、盐酸吡柔比星(THP)对LA-N-6的效应剂量,流式细胞术(FACS)及实时定量PCR（RT-PCR）测定Foxp3在LA-N-6中的表达和CTX、THP对LA-N-6中Foxp3表达的影响。结果：FACS结果显示LA-N-6细胞高表达Foxp3，加入 CTX、THP后显著降低了LA-N-6上Foxp3的表达（P<0.05）；RT-PCR显示CTX显著降低了LA-N-6细胞中Foxp3的表达（P<0.05），THP加药组与对照组比较差异无统计学意义。结论：肿瘤逃逸相关转录因子Foxp3高表达于神经母细胞瘤细胞LA-N-6；CTX、THP能分别在蛋白或基因水平降低Foxp3分子在LA-N-6的表达，提示CTX、THP可能通过抑制Foxp3分子的表达而抗肿瘤的作用。 ［中国当代儿科杂志，2010，12（5）：386-389］

Neuroblastoma LA-N-6 cells express Foxp3 which can be suppressed by chemotherapeutic agents

Objective To investigate whether neuroblastoma cells LA-N-6 express Foxp3 and whether the expression of Foxp3 is sensitive to chemotherapy by cyclophosvnamide(CTX)and pirarubicin(THP).Methods Expression of Foxp3 on LA-N-6 cells was examined by flow cytometry analysis.The dose-effects of chemotherapy drugs including CTX and THP on LA-N-6 cells were investigated by MTT assay.The effects of CTX and THP on Foxp3 expression were examined by flow cytometry and real-time PCR assays.Results Flow cytometry analysis ...