Unusual SCD in cancer cells: A phenomenon related to decreased BrdU incorporation? |
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| Authors: | Z Gibas R Becher A A Sandberg J Limon U Bregula M Rochowska |
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| Institution: | 1. Department of Genetics and Endocrinology, Roswell Park Memorial Institute, Buffalo, NY USA;2. Genetics Laboratory, Medical Academy of Gdansk, Gdansk, Poland;3. Department of Tumor Biology, Institute of Oncology, Warsaw, Poland |
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| Abstract: | The simultaneous presence of chromosome segments with and without sister chromatid differentiation (SCD) in the same metaphase was observed in a human melanoma cell line after BrdU incorporation for 48-72 hr. This phenomenon was related to the time of BrdU incorporation by the cells: i.e., it was not observed after exposure to BrdU for only 17-20 hr. It is proposed, therefore, that the unusual staining pattern is caused through reduced BrdU incorporation by the cells after completing the first division. |
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