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Siliconizing glassware to be used for suspension cell culture
Authors:William D. Noteboom  Peter C. Will
Affiliation:(1) Department of Biochemistry, University of Missouri Medical School, 65201 Columbia, Missouri, USA;(2) Present address: Department of Anatomy, Case Western Reserve University, 44106 Cleveland, OH, USA
Abstract:Summary Siliconizing glassware to be used for suspension cell culture with the reagent dichlorodimethylsilane after rigorous cleaning of the glass results in a more homogenously treated glass which will survive many detergent washings. The method described was found to be superior to other ways of coating culture glassware.
Keywords:suspension culture  dichlorodimethylsilane
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