纳米复合烤瓷材料的生物相容性试验

目的：比较纳米复合烤瓷、普通烤瓷和纯钛3种修复材料的细胞毒性，初步评价纳米复合烤瓷材料的生物相容性。方法：采用体外细胞培养的四唑盐比色试验（MTT assay）和材料浸渍液培养细胞的形态学观察法。结果：3种修复材料在试验中的吸光度值无显著性差异（P＞0.05），毒性均为0级；倒置相差显微镜观察细胞形态，未发现异常。结论：纳米复合烤瓷材料有良好的细胞相容性，是一各上有应用前景的口腔修复材料。

An Experimental Study on the Biocompability of Porcelain Compounded With Nanostructured Material

Objective:Our aim was to evaluate the biocompability of porcelain compounded with nanostructured material. Methods:The proliferation of the NIH3T3 cells were investigated using a tetrazolium based colorimetric assay(MTT assay).Maceration extract of four tested materials were added into culture plate .The samples were tested with ultraviolet spectrophotometer in 490 wavelength at 1,3,5,7 days after the addition of extract. Morphology of these cells was also observed with phase contrast microscope. Results: There was no obvious difference among three kinds of prosthetic materials in the optical absorbance and the cells growing in the extract showed normal morphology. Conclusion: The toxicity graduation of the porcelain compounded with nanostructured material is 0. This material has a good biocompability and can be used clinically.