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纳米羟基磷灰石/细菌纤维素复合材料及其降解物的细胞相容性评价
引用本文:郑琪,奚廷斐. 纳米羟基磷灰石/细菌纤维素复合材料及其降解物的细胞相容性评价[J]. 中国神经再生研究, 2010, 14(3): 405-409
作者姓名:郑琪  奚廷斐
作者单位:中国药品生物制品检定所,北京大学前沿交叉学院
基金项目:国家重点基础研究发展计划(973计划);国家高技术研究发展计划(863计划)
摘    要:背景:新型复合材料纳米羟基磷灰石/细菌纤维素是一种极具应用前景的骨组织工程材料,而骨组织工程材料要求其本身及其降解产物具有良好的细胞相容性,实验在传统的MTT法评价细胞相容性的基础上,进一步应用流式细胞术的方法从DAN合成周期的角度进行评价。目的:评价新型纳米复合材料纳米羟基磷灰石/细菌纤维素及其酶降解产物的细胞相容性。 方法:应用体外细胞培养法,观察纳米羟基磷灰石/细菌纤维素复合材料及其降解物对成骨细胞形态学的影响,同时采用 MTT 比色法评价纳米羟基磷灰石/细菌纤维素及其降解物对成骨细胞生长和增殖的影响,并尝试用流式细胞仪检测材料作用于细胞后细胞周期时相的变化,从而在分子水平上评价材料对细胞增殖的影响。结果与结论:纳米羟基磷灰石/细菌纤维素复合材料及其降解物对成骨细胞的形态无明显影响,对细胞生长和增殖无明显抑制作用。MTT 细胞毒性试验显示原材料及其降解物的细胞增殖率均在80%以上,细胞毒性均为1级,材料对培养细胞无明显细胞毒性。流式细胞仪检测结果显示材料与细胞接触后能降低G0/G1期细胞比例,增加S,G2/M期细胞比例,能增加成骨细胞DNA的合成,促进成骨细胞生长和组织修复。提示纳米羟基磷灰石/细菌纤维素复合材料细胞相容性良好,是一种安全的、很有应用前景的骨组织工程支架材料。关键词:细菌纤维素;羟基磷灰石;纳米复合材料;细胞毒性;细胞周期;相容性;成骨细胞doi:10.3969/j.issn.1673-8225.2010.03.007

关 键 词:纳米复合材料;细胞毒性;细胞周期;相容性;成骨细胞
修稿时间:2010-01-06

Cytocompatibility of nano-hydroxyapatite/bacterial cellulose nanocomposites and its residues
zheng qi and Xi Tingfei. Cytocompatibility of nano-hydroxyapatite/bacterial cellulose nanocomposites and its residues[J]. Neural Regeneration Research, 2010, 14(3): 405-409
Authors:zheng qi and Xi Tingfei
Affiliation:National Institute for Control of Pharmaceutical and Biological Products,Academy for Advanced Interdisciplinary Studies,Peking University
Abstract:BACKGROUND: The nano-hydroxyapatite/bacterial cellulose (nHAP/BC) nanocomposites has a good prospect of application in bone tissue engineering, and the bone tissue engineered materials and its degradation products should have excellent compatibility. This study further assessed DAN synthesis cycle using flow cytometry on the basis of evaluating cell compatibility by metabolic 3-( 4, 5-dim ethylthiazo l-2-y l) -2, 5-Dipheny l-2H-tetrazolium (MTT) assay. OBJECTIVE: To evaluate the cytocompatibility of a new-pattern nHAP/BC nanocomposites and its residues. METHODS: Effects of nHAP/BC nanocomposites and its residues on morphological changes in osteoblasts were observed using in vitro cell culture method. Effects of nHAP/BC nanocomposites and its residues on osteoblast growth and proliferation were evaluated by MTT assay. Cell cycle phase changes were detected using flow cytometry to evaluate material effects on cell proliferation on molecular levels. RESULTS AND CONCLUSION: The nHAP/BC nanocomposites and its residues had neither remarkable effects on cell morphology, nor significant inhibition on osteoblast growth and proliferation. Test of MTT cytotoxicity showed that the average cell proliferation rate was over 80% after treated with the material and its residues, with the cytotoxity grade of 1 (non-toxic). Flow cytometry indicated that the rate of G0/G1 was reduced, and the rates of S, G2/M were increased, and the synthesis of DNA was increased, the cellular growth and repair in osteoblasts was accelerated. These indicated that nHAP/BC nanocomposites have good cytocompatibility, and it will be safe and prospected scaffolds in bone tissue engineering.
Keywords:Nanocomposites   Cytotoxicity   Cell cycle   Compatibility   Osteoblasts
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