乙型肝炎病毒DNA定量检测及其意义

目的　探讨乙型肝炎患者血清中HBV -DNA含量与HBV血清学标志物 (HBV -M)的关系。方法　采用荧光探针定量聚合酶链反应 (PCR)方法检测 2 0 8份不同临床类型血清标本的HBV -DNA含量 ,再用酶联免疫吸附试验 (ELISA)测定HBV -M ,总结二者的关系。结果　在HBsAg和HBeAg阳性的血清中HBV -DNA含量最高 ,血清HBeAg和HBV -DNA含量密切相关 ,但一些HBeAg阴性的血清中仍可检出HBV -DNA。结论　定量PCR可真实反映HBV感染、复制及病情变化情况 ,有助于乙肝的预后和抗病毒疗效的监测。

Quantitative detection of HBV-DNA and its significance

Objective It is to discuss the relation between the serum HBV-DNA content and the serological marker of HBV (HBV-M). Methods HBV-DNA content was examined with fluorescence quantitative polymerase chain reaction (FQ-PCR) and HBV-M was detected with enzyme-linked immunosorbent assay (ELISA) in 208 serum specimens of patients with different clinical types. The relation between them was summarized. Results HBV-DNA contents in positive sera of both HBsAg and HBeAg were highest. There was close relativity between serum HBeAg and HBV-DNA. But HBV-DNA could also be detected in some negative sera of HBeAg. Conclusion Quantitative PCR can reflact the true state of HBV infection, replication and the change of disease, and is more helpful for prognosis and monitoring the efficacy of antiviral therapy of hepatitis B.