大鼠实验性胃癌菜豆凝集素亲合组织化学研究

本文采用ＡＢＣ亲合组织化学技术研究了５３例Ｗｉｓｔａｒ大鼠胃粘膜腺上皮的菜豆凝集素（ＰＨＡ）受体的分布，其中正常大鼠胃粘膜１７例，腺胃胃癌２２例，异型增生１４例。结果显示大鼠胃粘膜表面上皮不结合ＰＨＡ，胃底腺与幽门腺的结合阳性率各为８９．８％与２３．５％。结合反应从界嵴循胃底腺至胃窦幽门腺、从胃底腺的颈部到体部再到底部均呈逐渐减弱趋势，而幽门腺的颈部和体部腺上皮ＰＨＡ反应强度高于腺底部细胞。大鼠腺胃幽门部胃癌及异型增生腺体的结合阳性率及阳性强度均高于相应部位的正常胃粘膜，且阳性主要定位于癌变腺体的腔膜面或核上区胞浆。结果表明，大鼠正常胃粘膜ＰＨＡ受体的分布有一定的规律性，大鼠实验性胃癌表现有与人类胃癌相似的病理生物学特征。

^The Study of PHA Affinity Histochmistry on ExperimentalGastric Carcinoma

We report the result of PHA receptor distribution on 53 cases of stomach mucosas of wistar rats in which 17 carcinomas and 14 cases of normal gastric mucosas, 22 cases of glanbular gastric carcinomas and 14 cases of dysplasia gastirc mucosas were included by use of ABC affinity histochemical technique.The superficial epithelium of normal gastirc mucosa in wistar rats didn't bind PUA,the positive rate of fundus gland and pyloric gland were 89.9% and 23.5% respectively,the positive reaction was distributed diffusely in cytoplasm.Observing from fundus gland to pyloric gland; and from neck, body to bottom of fundus gland,the intensity of PHA binding reaction was weaken progressively,while the reactive intensity of PHA in pyloric gland was neck = body>bottom. Both of the positive rate and staining intensity of gastric carcinoma and dysplasia in pyloric mucosa were higher than corresponding normal gastric mucosa. The PHA binding reaction was mainly distributed along luminal surface of the glands or in distribution of PHA receptor on normmal gastric mucosa of rats has a certain regularity and the experimental gastric carcinoma in rats has the same pathobiological feature as the human gastric carcinoma.