HLA-DR基因与广西钦州市人群肺结核的相关性研究

目的探讨人类白细胞抗原HLA-DR等位基因与广西人群肺结核发病相关性,寻找与肺结核发病可能相关的HLA-DR易感基因。方法采用病例-对照研究方法,应用聚合酶链反应-序列特异性引物（PCR-SSP）技术对100名肺结核患者和105名健康对照者的HLA-DR等位基因进行分型,比较其等位基因频率（GF）并计算其比值比（OR）。结果肺结核病组中的HLA-DRB1＊14频率（12.0%）明显高于对照组（3.8%）,其差异具有显著统计学意义（χ2=4.775,P=0.029,OR=3.443）;肺结核病组中的HLA-DRB1＊13的频率（23.0%）明显低于对照组（42.9%）,其差异亦具有显著统计学意义（χ2=9.111,P=0.003,OR=0.398）。结论 HLA-DRB1＊l4基因可能与广西人群肺结核的发病密切相关,而HLA-DRB1＊13基因可能对广西人群肺结核发病具有拮抗作用。

Correlation between HLA-DR genes and susceptibility to pulmonary tuberculosis in population of Qinzhou city in Guangxi province

Objective To analyze the association between HLA-DR genes and pulmonary tuberculosis and explore susceptible genes associated with pulmonary tuberculosis in the population in Qinzhou City in Guangxi province.Methods Using case-control study,we detected the alleles of HLA-DR gene sites in l00 tuberculosis cases and 105 controls from Qinzhou city in Guangxi provinces by polymerase chain reaction-sequence specific primers（PCR-SSP）technique.Gene frequency（GF） and odds ratio（OR） were calculated and compared.Results The frequency of HLA-DRB1＊14 in pulmonary tuberculosis cases was strikingly higher than that in the healthy controls（χ2= 4.775,P=0.029,OR=3.443）,their GFs were 12.0% and 3.8% respectively;significantly decreased frequency of HLA-DRB1＊13 in pulmonary tuberculosis cases was found（χ2=9.111,P=0.003,OR=0.398）,their GFs were 23.0% and 42.9% respectively.Conclusion HLA-DRB1＊14 is closely correlated to incidence of pulmonary tuberculosis in the population of Qinzhou in Guangxi,and expression of HLA-DRB1＊13 may be associated with an antagonist effect in the pathogenesis of pulmonary tuberculosis in this population.