| 变形链球菌和远缘链球菌耐氟菌株DNAG+C含量测定 |
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| 引用本文： | 盛江筠,刘正. 变形链球菌和远缘链球菌耐氟菌株DNAG+C含量测定[J]. 上海口腔医学, 2001, 10(1): 49-51 |
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| 作者姓名： | 盛江筠 刘正 |
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| 作者单位： | 上海第二医科大学附属第九人民医院口腔医学院 口腔内科, |
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| 基金项目： | 国家自然科学基金(39870780) |
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| 摘 要： | 目的 探讨变形链球菌和远缘链球菌耐氟菌株基因型是否发生改变。方法 采用高效液相色谱法测定变形链球菌和远缘链球菌及相应耐氟菌株DNA碱基含量，即DNAG＋C摩尔百分比(mol%)。结果 四株耐氟菌株DNAG＋Cmol%为54.45,44.86,54.41,55.39；相应亲代菌株G+Cmol%为35.8,37.4,43.35和46.62。两者明显不同，差值大于5％。结论 变形链球菌和远缘链球菌的耐氟菌株已发生基因型改变，且可能包括多个基因的突变。
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| 关 键 词： | 耐氟菌株 变形链球菌 远缘链球菌 DNA G＋Cmol% 基因型 |
| 文章编号： | 1006-7248(2001)01-0049-03 |
| 修稿时间： | 1999-10-05 |
Base Composition of DNAfrom Fluoride-Resistant Strains of Streptococcus Mutans and Streptococcus Sobrinus |
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| SHENG Jiang yun,LIU Zheng.. Base Composition of DNAfrom Fluoride-Resistant Strains of Streptococcus Mutans and Streptococcus Sobrinus[J]. Shanghai journal of stomatology, 2001, 10(1): 49-51 |
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| Authors: | SHENG Jiang yun LIU Zheng. |
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| Affiliation: | School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China. |
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| Abstract: | Objective To evaluate the genotype change of fluoride resistant strains of S.mutans and S.sobrinus. Methods High potential liquid chromatography was used to measure the base composition of DNA from F resistant strains of S.mutans and S.sobrinus and their parent strains. Results The data showed that the DNA G C mol% of F resistant and F sensitive S.mutans were quite different, the difference was over 5%. The four F resistant strains were 54.45,44.86,54.41,55,390, whereas the corresponding four parent strains were 35.38,37.4,43.35,46.62, respectively. Conclusion The genotypes of F resitant strains of S.mutans and S.sobrinus have been changed and may comprise several gene mutations. [ |
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| Keywords: | F resistant Strain Streptococcus Mutans Streptococcus Sobrinus DNA G C mol% Genotype |
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