骨髓间充质干细胞移植对大鼠重症急性胰腺炎的治疗作用观察

目的探讨骨髓间充质干细胞（MSCs）移植对重症胰腺炎（SAP）的治疗作用及相关机制。方法将雄性SD大鼠120只随机分为假手术组、模型组及移植组各40只。模型组和移植组采用5%牛磺胆酸钠逆行胰胆管注射方法制作SAP模型,假手术组开腹后翻动肠壁即关腹。造模成功后移植组经门静脉植入MSCs;模型组及假手术组采用同样方法给予等量生理盐水。于24、48、72 h每组分别处死10只大鼠,对胰腺和肾脏损伤进行大体形态和光镜下观察,测定血清淀粉酶（AMY）、BUN、Cr、TNF-α和IL-6水平。结果模型组胰腺和肾脏组织损伤明显,各时间点血清AMY、BUN、Cr、TNF-α和IL-6水平均高于假手术组,P均〈0.05。移植组胰腺和肾脏组织损伤有所减轻;各时间点血清中AMY、BUN、Cr、TNF-α和IL-6水平均低于模型组及假手术组,P均〈0.05。结论经门静脉移植MSCs治疗SAP效果确切,可降低多器官功能障碍的发生率。

Treatment effect of bone marrow mesenchymal stem cells transplantation on the severe acute pancreatitis in rats

Objective To study the protective effect and the possible mechanisms of transplantation bone marrow mesenchymal stem cells（ MSCs） on pancreatic injury in rats with severe acute pancreatitis（ SAP）. Methods A total of 120 male Sprague-Dawley rats were randomly divided into sham operation group（ N group）,severe acute pancreatitis group（ P group）,transplantation MSCs group（ M group）. The dose of 5% sodium taurocholate was retrograde injected into the pancreatic duct of the rats in P group and M group to induce SAP model. Rats in N group whose pancreases were just flipped and striked gently without injection. MSCs were transplanted into rats in M group via portal vein,while the same dosage of0. 9% NS was injected by the same vein routine in P group. Ten rats in each group were sacrificed at 24 h,48 h,72 h later. Pancreas and kidney damage of each group were observed under gross morphology and optical lens. The serum AMY,BUN,Cr,TNF- α and IL-6 level of each group were tested. Results Pancreas and kidney injury in P group were significantly heavier. The content of serum AMY,BUN,Cr,TNF- α and IL-6 level in P group were both higher than those of N group（P〈0. 05）. Pancreatic and renal tissue injury in M group rats mitigated. The content of serum AMY,BUN,Cr,TNF- α and IL-6 level in M group were both lower than those of P group（P〈0. 05）. Conclusions Transplantation via portal vein MSCs is an effective treatment on SAP,which can reduce the occurrence of multiple organ dysfunction and improve prognosis.