骨形态发生蛋白2在骨髓间充质干细胞向肝细胞分化中的作用

目的：观察骨形态发生蛋白2（BMP2）在骨髓间充质干细胞（BMSCs）向肝细胞分化中的作用。方法采用贴壁法分离培养大鼠股骨BMSCs ，将体外扩增的第3代BMSCs制作细胞爬片，并行肝细胞定向诱导。根据诱导因子不同分为：肝细胞生长因子（ HGF）组、HGF＋BMP2组、BMP2组及空白对照组。培养10 d左右收集细胞，观察各组细胞形态的变化，并采用ELISA法检测培养液上清中肝细胞特异性标志物甲胎蛋白（ AFP）、白蛋白（ ALB），免疫细胞化学法检测诱导分化后细胞CK-18的表达。结果 HGF组和HGF＋BMP2组可检测到ALB、AFP及CK-18，且HGF＋BMP2组ALB、AFP及CK-18明显高于HGF组（P均＜0．05）。结论 BMP2不能单独诱导BMSCs向肝细胞分化，但能增强HGF诱导BMSCs向肝细胞分化的作用。

Effect of BMP2 on mesenchymal stem cells differentiating into hepatocytes

Objective To observe the effect of BMP2 on bone marrow mesenchymal stem cells （BMSCs） differentia-ting into hepatocytes .Methods BMSCs in thighbone of rats were isolated and cultured using adherent culture method . The third generation of amplification of BMSCs in vitro was made into cell climbing piece , and directional inducting hepato-cyte.BMSCs was divided into Hepatocyte growth factor （HGF） group , HGF ＋BMP2 group, BMP2 group and blank con-trol group depending added inducing factor .Cells were collected after culturing about 10 days.Changes in cell morphology was observed .Using enzyme-linked immunosorbent assay to culture supernatant of liver cell-specific marker alpha-fetopro-tein （AFP）, albumin （ALB）, Cytokeratin immunohistochemistry assay cells -18 （CK-18）.Results ALB, AFP and CK-18 in HGF group and HGF ＋BMP2 group can be detected , and were higher than those in HGF ＋BMP2 group （ all P〈0.05）.Conclusion BMP2 can not alonely induce BMSCs differentiating into hepatocytes ,enhance the effect of HGF-in-duced hepatocyte differenting into BMSCs .