缺氧缺血性脑损伤大鼠脑组织海马区 p-tau 蛋白与凋亡相关蛋白 Bcl-2、Bax 的关系 |
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引用本文: | 王军,陈静,张冬秀,王宝珍,朱登纳,牛国辉,程萍萍. 缺氧缺血性脑损伤大鼠脑组织海马区 p-tau 蛋白与凋亡相关蛋白 Bcl-2、Bax 的关系[J]. 山东医药, 2014, 0(45): 1-3 |
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作者姓名: | 王军 陈静 张冬秀 王宝珍 朱登纳 牛国辉 程萍萍 |
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作者单位: | 郑州大学第三附属医院,郑州450052 |
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基金项目: | 河南省教育厅科学技术研究重点项目(14A320004). |
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摘 要: | 目的:观察缺氧缺血性脑损伤(HIBD)大鼠脑组织海马区磷酸化tau(p-tau)蛋白及凋亡相关蛋白Bcl-2、Bax的表达,并分析其相关性。方法选择新生SD大鼠56只,随机分为正常对照组8只、假手术组8只和HIBD组40只(按处死时间点分为3、6、12、24、48 h 5个亚组,每组8只)。 HIBD组参照Rice法建模,并在不同时间点断头取脑。假手术组仅暴露左颈总动脉,正常对照组未做处理,均于手术结束后断头取脑。采用HE染色法观察各组脑组织海马区细胞形态结构改变,免疫组化染色法观察各组脑组织海马区p-tau、Bcl-2、Bax蛋白阳性细胞表达。结果正常对照组及假手术组脑组织细胞形态结构正常,基本无损伤性改变。 HIBD组在HIBD后3 h细胞无明显变化;HIBD后6 h出现细胞肿胀,结构排列紊乱;HIBD后24 h细胞肿胀明显,细胞核固缩、碎裂;HIBD后48 h细胞内出现凋亡小体,神经细胞数量锐减。 HIBD组各时间点脑组织海马区p-tau、Bcl-2、Bax蛋白阳性细胞数均较正常对照组和假手术组显著增多(P均<0.05);HIBD后12 h内,脑组织海马区p-tau蛋白阳性细胞数与Bcl-2、Bax蛋白阳性细胞数均呈正相关( P均<0.05)。结论 HIBD后脑组织海马区细胞启动了凋亡程序,p-tau蛋白参与了海马区细胞的凋亡过程,并且与Bcl-2、Bax蛋白的表达存在相关性。
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关 键 词: | 缺氧缺血 脑 p—tau蛋白 细胞凋亡 Bcl-2 Bax 大鼠 |
The connection among the expression of p-tau protein and apoptosis related proteins Bcl-2 and Bax in the hippocampus of hypoxic-ischemic neonatal rats |
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Affiliation: | WANG Jun, CHEN Jing, ZHANG Dong-xiu, WANG Bao-zhen, ZHU Deng-na, NIU Guo-hui, CHEGN Ping-ping ( The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China) |
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Abstract: | Objective To observe the expression of phosphorylated tau ( p-tau) protein, and apoptosis related pro-teins Bcl-2 and Bax, and explore their correlations .Methods Seven-day-old newborn clean Sprague Dawley ( SD) rats, were randomly divided into normal control group (8 cases), sham operation (8 cases), and HIBD group (which was divid-ed into by death time points 3, 6, 12, 24, 48 h subsection 8 cases in each group).HIBD animal model was established by Rice method, and took the brain at different time points .In sham group, rats were just exposed the left carotid artery , and in the control group rats did not dispose .Rats were all decapitated brain immediately at the end of surgery .Hematoxylin-e-osin ( HE) staining was used to view morphological changes in the hippocampus , and immunohistochemical staining was used to test the expressions of p-tau, Bcl-2 and Bax protein.Results Brain tissues in the normal control group and sham operation group was normal .In HIBD group, the cell has not significant change 3 h after HIBD;Cell swelling and structur-al disorder 6 h after HIBD; Cell swelling significantly , nuclear condensation , fragmentation 24 h after HIBD;48 h after HIBD, apoptotic bodies occured , the number of nerve cells was dropped .In HIBD group, Bcl-2 and Bax positive cells in HIBD group at each time point were more than those in the normal control group and the sham group (all P〈0.05);In the hippocampus of HIBD group , p-tau positive cells was positively correlated with Bcl-2 and Bax positive cells during 12 h af-ter HIBD(P〈0.05).Conclusion The hippocampus neurolcytes actiwated apotosis mechanism afer HIBD early period . P-tau protein was involved in neuronal apoptosis after HIBD , and there is connection among p-tau, Bcl-2 and Bax protein. |
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Keywords: | hypoxic-ischemia, brain p-tau protein apoptosis Bcl-2 Bax rat |
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