The evidence of human herpesvirus 6 infection in the lymph nodes of Hodgkin's disease |
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Authors: | Akihiko Maeda Tetsutaro Sata Hideaki Enzan Keiko Tanaka Hiroshi Wakiguchi Takanobu Kurashige Koichi Yamanishi Takeshi Kurata |
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Affiliation: | (1) Department of Pathology, National Institute of Health, Toyama 1-23-1, Shinjuku-ku, 162 Tokyo, Japan;(2) Department of Pathology, Kochi Medical School, Nankoku, 783 Kochi, Japan;(3) Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, 565 Osaka, Japan;(4) Department of Pediatrics, Kochi Medical School, Nankoku, 783 Kochi, Japan |
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Abstract: | Human herpesvirus 6 (HHV-6), the causative agent of exanthem subitum, has been implicated in other diseases. Recently HHV-6-specific sequences have been detected by Southern blot analysis and polymerase chain reaction in the lymph nodes of three patients with Hodgkin's disease. The pathological localization of HHV-6, however, is still unknown. In order to study the pathological role of HHV-6 in Hodgkin's disease, we investigated, by immunohistochemical and molecular methods, two lymph node biopsies taken from a 7-year-old boy with Hodgkin's disease during the course of disease evolution. Although the histopathological findings of the first biopsy differed from those of the second, HHV-6 antigens and sequences could be detected in both lymph nodes by immunohistochemistry and in situ hybridization, respectively. HHV-6 was localized in macrophages, predominantly in lymphoid follicles, but not in ReedSternberg cells. Antibody titres to HHV-6 were consistent with reactivation of latency. Neither cytomegalovirus nor Epstein-Barr virus was present. Our data suggest a role for HHV-6 in the pathogenesis of Hodgkin's disease. |
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Keywords: | Human herpesvirus 6 Epstein-Barr virus Hodgkin's disease In situ hybridization Immunohistochemistry |
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