扇贝裙边糖胺聚糖的体外抗肿瘤活性及对荷瘤小鼠抗氧化作用的研究

目的:研究扇贝裙边糖胺聚糖(SS-GAG)的体外抗肿瘤活性及对荷瘤小鼠的抗氧化功能的影响。方法:用MTT法观察不同浓度的SS-GAG对体外培养的宫颈癌HeLa、人大肠癌LOVO、肺癌A549、神经胶质瘤U251等肿瘤细胞株增殖活性的影响;观察SS-GAG对小鼠接种S180后30d内的存活时间;通过黄嘌呤氧化酶法、硫代巴比妥酸显色法等多种生化方法观察SS-GAG对荷瘤小鼠血清总抗氧化能力(T-AOC)、超氧化物岐化酶(SOD)活性和丙二醛(MDA)含量的影响,并与对照比较。结果:与对照比较,SS-GAG可明显抑制HeLa、U251细胞生长,对LOVO、A549细胞生长也有一定的抑制作用;SS-GAG能显著延长S180腹水瘤小鼠的生存时间,提高荷瘤小鼠的T-AOC、SOD水平,降低MDA含量(P<0.01或P<0.05)。结论:SS-GAG可抑制肿瘤生长,并有一定的抗氧化作用。

Antitumor Activity in Vitro and Antioxidation in Vivo on Tumor- bearing Mice of Glycosaminoglycan from Scallop Shirt

OBJECTIVE ： To study the antitumor activity and antioxidative action of glycosaminoglycan from scallop skirt （SS- GAG） . METHODS： The effects of different concentration of SS- GAG on the proliferation of four tumor cell lines （HeLa, LOVO, A549, and U251） which had been cultured in vitro were observed by MTT. The effect of SS-GAG on mice within 30 d after innoculation of sarcoma 180 was observed. Multi - kind of biochemistry methods including xanthine oxidase method and thiobarbituric acid colorimetric method were used to study the effect of SS- GAG on total antioxidation activity, activity of SOD, and level of MDA in tumor - bearing mice, which were compared with those in control group. RESULTS： As compared with control group, SS - GAG significantly inhibited the growth of HeLa and U251 cell lines, inhibited the growth of LOVO and A549 cell lines to some degree, significantly prolonged the survival time of the mice with S180 ascites tumor, en- hanced the total antioxidant activity and the SOD activity and decreased MDA content in serum（P〈0.01 or P〈0.05） . CONCLUSIONS： SS- GAG has inhibitory effect on tumor and certain antioxidant activity.