HPLC法检测冬凌草甲素壳聚糖微球包封率及载药量 |
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引用本文: | 何洁,李俊良,王琴,李黛娜.HPLC法检测冬凌草甲素壳聚糖微球包封率及载药量[J].浙江中医药大学学报,2013(11):1326-1329,1335. |
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作者姓名: | 何洁 李俊良 王琴 李黛娜 |
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作者单位: | 浙江中医药大学生物工程学院杭州310053 |
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基金项目: | 基金项目:2012年浙江省自然科学基金项目(LQ12H28006);2012年浙江省大学生科技创新活动计划(新苗人才计划)(2012R410026):2011年浙江中医药大学校级科研基金(2011ZZ12);浙江省医药卫生科学研究基金计划(20098115) |
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摘 要: | 【目的]建立冬凌草甲素壳聚糖微球载药量和包封率测定的高效液相色谱分析方法。【方法]采用酸回流破乳法对制得的冬凌草甲素壳聚糖微球进行破乳,采用高效液相色谱法测定微球的载药量和包封率。色谱条件:流动相:甲醇-水(50:50);Kromasil C18柱(50mm×4.6mm,5μm);流速:1.0mL/min;检测波长:238nm;柱温:30℃。结果】当冬凌草甲素投药量与载体的质量比为4:10时,载药量为2.238%,包封率为0.789%,达到最高值。结论】此色谱奈件对冬凌草甲素的检测具有良好的稳定性。
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关 键 词: | 冬凌草甲素 高效液相色谱法 载药量 包封率 |
Study on HPCL Detection for Drug Content and Entrapment Efficiency of Oridonin Chitosan Microsphere |
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Institution: | He Jie, Li Junliang, Wang Qin, et al College of Bioengineering Zhejiang Chinese Medical University, Hangzhou(310053) |
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Abstract: | Objective]To establish HPLC method to determine drug content and entrapment efficiency oforidonin chitosan microspheres. Method] Oridonin chitosan microspheres were broken by acid reflux,drug content and entrapment efficiency of the microsphere were detected by HPLC. The chromatographic conditions: methanol : water (50 : 5(I) as mobile phase, the separation was performed oll a Kromasil C18 column(50 mm×4.6 mm, 5 μm) at a flow rate of 1.0 mL/min. The detection wavelength was 238 nm and the column temperature was 30℃ Results]The drug cont, at oforidonin chitosan microphere was 2.238%, the encapsulated efficency was (3.789 %.Conclusion]There is good stability detection of oridonin in chromatographic condition. |
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Keywords: | oridonin HPLC drug content entrapment efficiecny |
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