用胚胎干细胞诱导的造血干/祖细胞重建小鼠造血功能研究

目的 胚胎干细胞体外诱导分化出现CD34^ 造血干/祖细胞，然后将其注射入致死量照射小鼠体内，以研究其重建造血功能。方法 胚胎干细胞自由分化形成胚胎体后，加入造血刺激因子混合液以诱导产生CD34^ 造血干／祖细胞，将这些造血干／祖细胞注射入经致死剂量照射的小鼠，观察小鼠存活率的改变，并取存活2个月的小鼠骨髓和脾脏，PCR检测嵌合体形成。结果 体外分化第13日CD34^ 造血干／祖细胞比例可高达17．36％，将这些细胞注射入致死量照射小鼠后，可提高存活率达86．67％，存活小鼠的骨髓和脾脏均检测到供体来源的细胞。结论 胚胎干细胞体外分化获得CD34^ 造血干/祖细胞，且后者具有其相应的正常生物学功能。

Reconstitute of the Hematopoietic Function of Mice Using Hematopoietic Stem/Progenitor Cells Derived from Embryonic Stem Cells

Objective To induce Embryonic stem cells to differentiate into CD34+ hematopoietic stem/progenitor cells in vitro. To reconstitute the hematopoietic function of lethally irradiated mice. Methods Embryonic stem cells, formed embryoid bodies, were induced to differentiate into CD34+ hematopoietic stem/progenitor cells when cultured with hematopoietic growth factors. These CD34+ hematopoietic stem/progenitor cells were injected into lethally irradiated mice. PCR was performed to detect the sex-determining region of the Y-chromsome derived from the male donor cells in the bone marrow and the spleen cells of the transplanted female mice. Results The percentage of CD34+ cells could reach 17.36% on the thirteenth day. The survival rate of the group transplanted with CD34+ hematopoietic stem/progenitor cells was 86.67% . The donor cells were both found in the bone marrow and the spleen cells of the survived transplanted mice. Conclusion Embryonic stem cell could be induced to differentiate into CD34+ hematopoietic stem/progenitor cells in vitro, and these cells could reconstitute the hematopoietic function of the lethally irradiated mice.