二甲基亚硝胺致大鼠肝纤维化的造模研究

采用二甲基亚硝胺对大鼠进行急性小剂量间歇性腹腔注射,制作实验性肝纤维化模型。结果发现,在染毒期间大鼠肝内小叶中央出血性变性坏死的炎性浸润持续存在,肝细胞外基质进行性增加,染毒4周末在肝内形成中心—中心性和/或中心—门脉性纤维间隔。停止染毒后4周末肝内出血性病变基本消失,炎性浸润明显减轻,但纤维化仍然明显。血清和肝脏生化检测等结果与肝内病理改变相一致.本模型造模周期短,大鼠死亡率低,肝纤维化形成相对稳定,值得在中医药抗肝纤维化研究中使用。

A Modeling Study of Dimethylnitrosamine-induced Liver Fibrosis in Rats

With four weeks of intermittent intraperitoneal exposure of Dimethylnitrosamine (DMN) which has hepatotoxicity, genotoxicity and immunotoxicity, an experimental model of liver fibrosis in rats had been produced. It was found that in rats DMN exposure led to hepatic centrolobular hemorrhagic necrosis, Iymphomonocytic infiltration, consequentially mesenchymal hyperplasis and eventually formation of central-central and / or central-portal fibrotic septa by the end of the fourth week. It was alsc found that four week after the discontinuation of DMN exposure, liver fibrosis was still conspious, meanwhile, lymphomono-cytic infiltration was lessen significantly and hemorrhagic necrosis almost disappeared. The data from biochemical measurements of blood and liver accorded with hepatic pathologic changes. Because of its shorter term and lower mortality of modeling, relative stablity of li ver fibrosis and similarity with pathologic changes of human liver disease, it is valuable in the study of the effects of traditional Chinese herbs on liver fibrosis.