A simple method for multiple fluid exchange |
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Authors: | M. Ritter F. Lang |
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Affiliation: | (1) Institute for Physiology, University of Innsbruck, Fritz-Pregl-Strasse 3, A-6010 Innsbruck, Austria |
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Abstract: | A simple device for fluid exchange is described, which allows the exchange of an unlimited number of solutions at low (10–1000 nl/min) constant perfusion rate. The applicability of the system has been tested in microperfusion experiments of rat distal tubules. At a luminal perfusion rate of 40 nl/min, the lag time was some 20 sec and 80 % fluid exchange time some 3 sec. Simple modification allows further reduction of the lag time. Under control conditions, the potential difference across the late distal tubule (PDte) approaches –19.4±2.5 mV (n = 27). Increase of luminal potassium concentration from 5.4 to 40 mmol/l hyperpolanzes PDte to –29.9±4.3 mV (n = 8). Amiloride (10 mol/l) leads to a reversible depolarization to –3.2±1.0 mV (n = 19), barium (1 mmol/l) to a reversible hyperpolarization to –25.8±2.6 mV (n = 19). As expected, PDte is largely created by amiloride sensitive sodium channels and is partially blunted by barium sensitive potassium channels. |
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Keywords: | Distal renal tubule Microperfusion Amiloride Barium Potential difference |
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