Studies on the flow and distribution of leukocytes in mesentery microcirculation of rats

AIM To study the effect of leukocyte-endothelium interaction (LEI) on the flow and distribution of leukocytes in microcirculation under physiological condition.METHODS microcirculation image multiple parameter computer analysis system (MIMPCAS) was used to study the flow and distribution of leukocytes in mesentery microcirculation of rats in vivo.RESULTS　he difference of visible leukocyte flux (VLF) was as high as 131 times in the arterioles and venules with similar diameter and blood velocity. The visible leukocytes rolled along the blood vessel wall as a “jerky” movement. The frequency distribution of the visible leukocyte velocity (VLV) showed a “two-peak” curve. The low peak value was at 10μm/s-15μm/s while the high peak fell between 25μm/s-30μm/s. With the increase of diameter of venules, VLF increased while the VLV remained at the same level. With the increase of RBC velocity, VLV trends to elevate and VLF to fall down.CONCLUSION The results herein might provide a basic theory for the study on the mechanism of LEI under physiological condition and novel methods for the prevention and treatment of high LEI in many pathological processes.