hCG抗原／缓释微球对C_（57）小鼠免疫应答的研究

本文描述了常规免疫ｈＣＧ和一次性接种ｈＣＧ／缓释微球（ｈＣＧ／ＰＧＬＡ）对Ｃ５７ＢＬ小鼠产生免疫应答的比较研究，对它们所产生的抗ｈＣＧ血清作了三种参数的比较：１．抗ｈＣＧ血清效价的动态变化；２．抗体在体内体外中和ｈＣＧ生物活性的能力；３．抗原抗体亲和常数的测定。结果表明：一次性接种ｈＣＧ／缓释微球能在Ｃ５７小鼠体内产生高效价的ｈＣＧ抗体，对三种参数的分析表明：一次性接种ｈＣＧ／缓释微球系统所产生的抗血清和常规免疫ｈＣＧ所产生的抗血清基本上是相似的，抗体浓度与中和ｈＣＧ生物活性的能力成正比。本研究提示：一次性接种疫苗的缓释微球完全可以代替普通疫苗的常规免疫方法，为今后的β－ｈＣＧ避孕疫苗和其它疫苗的研究和临床推广应用提供了新的便利方法。

The Study of Immunological Response of to Long-term Release System hCG Microsphere in C_(57) Black Mouse

This paper describes the study of the immunological response of C57 Black mouse to hCG by multiple inoculation with hCG and single injection with a long-term release system of hCG/poly (lactide-co-glycolide) microsphere (hCG/PGLA). Three parameters including 1. the dynamic variation of hCG antibody titres; 2. the capacity of hCG antibodies in vitro and in vivo and 3. the binding affinity constants of hCG antibodies were assayed. The results showed that the single injection with hCG/PGLA was capable of producing high titres of anti-hCG antibodies. The analyses of three parameters of antisera from C57 Black mouse showed that the properties of the antisera elicited using a single injection with hCG/PGLA were generally similar to that of the antisera raised by the multiple inoculations with hCG,and concentration of the antibodies were directly proportional to hCG bioneutralization capacity. Meanwhile,all of these results suggest us that a further long-term release system of β-hCG/PGLA as a vaccine may substitute for conventional vaccine and it also provide a new convenient inoculative method for widespread clinical application and research of hCG vaccine and other vaccine.