LHRH融合蛋白对雄性大鼠生殖功能的影响

目的 :以促黄体生成素释放激素融合蛋白 (Trx LHRH)为免疫疫苗 ,氢氧化铝为佐剂 ,研究Trx LHRH诱导雄性大鼠抗体生成及其对大鼠生殖功能的影响。　方法 :Trx LHRH为体外克隆融合基因在大肠埃希菌BL2 1表达的融合蛋白。以Trx LHRH为免疫疫苗 (0 .2mg)、氢氧化铝为佐剂 ,通过初次和加强免疫雄性成年SD大鼠 ,间接酶联免疫法测定抗体滴度 ,放射免疫法测定血清睾酮 (T)水平 ,同时观察附睾精子数量的变化。　结果 :Trx LHRH免疫 4周后抗体滴度达 1∶12 80～ 1∶2 5 6 0 ,加强免疫后直至 6周抗体滴度仍保持 1∶2 0 0 0高水平。血清T水平则显著降低 (P <0 .0 1) ,个体之间存在一定的差异 ;精子计数减少 (P <0 .0 5 )。　结论 :Trx LHRH能成功诱导大鼠产生抗LHRH抗体 ,并进而抑制垂体 睾丸轴的功能 ,抑制其生殖功能。

Effect of LHRH Crasis Protein as Vaccine on the Reproductive Function of Male Rats

OBJECTIVE: To investigate the effect of Trx-LHRH, a new GnRH crasis protein, on antibody production and male reproductive function. METHODS: Trx-LHRH produced in vitro with a new crasis gene which crasised Trx gene and GnRH gene together, was used as vaccine, and hydroalaminum base as adjuvant, in adult SD rats. After 5 weeks of the first treatment, the same dosage was used again to enhance the effect of vaccine. Antibody level was measured by ELISA, and androgen level by RIA. RESULTS: Trx-LHRH induced successfully the polycolonal antibody at the level of 1 :1 280 approximately 2 560 after 4 weeks of the first treatment, and 1 : 2 000 after 6 weeks of the enhanced treatment. Testosterone level was reduced significantly (P < 0.01) by ELISA, but there was reasonable variation among individuals. Sperm count was also reduced by Trx-LHRH treatment. CONCLUSION: Trx-LHRH can be used as effective vaccine to induce antibody production, and at the same time, restrain the function of hypothatamas-pituitary-testis axis in vivo.