大鼠心脏停搏供体肝移植的实验研究

目的 探讨大鼠原位肝移植过程中，供肝可能耐受心脏停搏热缺血损伤的时间极限，方法 雄性SD大鼠，以供肝获取前供体大鼠经历心脏停搏时间0、15、30、45、60min分为5组（HB组、N-15组、N-30组、N-45组和N-60组），而后行大鼠原位肝移植，比较各组的术后肝功能、肝脏病理和存活率。结果 HB、N-15、N-30、N-45和N-60的大鼠肝移植术后1周存活率分别为：100%（8/8）、75%（6/8）、62.5%(5/8)、25%（2/8）和0%（0/8）。其中HB、N-15、N-30组和N-45组的大鼠肝移植最长存活时间超过30d。结论 大鼠供肝对于热缺血损伤的时间应越短越好，但耐受30min心脏停搏热缺血损伤时，仍可使1周存活率达62.5%，极限时间为45min。

Study of rat liver transplantation from non-heart-beating donors

Objective To investigate the limiting tolerance to warm ischemia of liver grafts from non-heart-beating donors (NHBD).Methods Rat orthotopic liver transplantation was performed in 5 groups with stepwise of cardiac arrest time in the donor from zero to 60?min with 15?min intervals. Hepatic function, liver pathology and the rat survival rate in each group were compared.Results With the increasing duration of warm ischemia, one week survival rate of these 5 groups were 100%(8/8), 75%(6/8), 62.5%(5/8), 25%(2/8), and 0% (0/8) respectively. The recipients in the former four groups had the possibility to survive more than 30 days.Conclusions The liver is less sensitive to warm ischemia than formerly believed. Rat liver can be used for transplantation after cardiac arrest up to 30 min with a 62.5% of one-week survival rate. The maximal time using NHBD for rat liver transplantation was 45?min.