内毒素条件下血管内皮细胞对中性粒细胞粘附性的动态改变

应用细胞微管吸吮实验系统对内毒素刺激条件下牛肺动脉血管内皮细胞（ＶＥＣ）对中性粒细胞（ＰＭＮ）的粘附性行单细胞定量测量。结果发现：在内毒素刺激下，拉离粘附于ＶＥＣ表面的ＰＭＮ所需临界分离应力Ｓｃ由正常对照的０．０８６±０．００７ｋＰａ增加到０．２８３±０．０３１ｋＰａ，随刺激过程的延长，ＶＥＣ的粘附力呈继续升高状态，１２ｈ达高峰，２４ｈ仍无明显下降趋势。结果提示ＶＥＣ在内毒素刺激诱导下对ＰＭＮ粘附性的增加既有快相反应，又有慢相反应。这种粘附性的明显增加为内毒素性损伤脏器微循环中大量ＰＭＮ滞留现象的发生提供保证

Monocellular Quantitative Analysis of Vascular Endothelial Cell Adhesion to Polymorphonucler Neutrophil under Endotoxin Condition

A micromanipulation technique was used to determine the adhesive ability of vascular endothelial cell (VEC) to Polymorphonucler neutrophil (PMN) under endotoxin condition. By the stimulation of endotoxin, the force required to separate the PMN from VEC increased from 0.086 ± 0.007 kPa to 0.283 ± 0.031 kPa within 30 min. It still increased continously in later period, and achieved the peak value 0.983 ± 0.089 kPa in 12 h and had no tendency to decrease by 24 h. The results suggest that the increase of VEC stickness to PMN belonged to both rapid response and delayed response. It gives better elucidation of tremendous PMN sequestration in microcirculation of injured tissue under the endotoxemia conditions.