Changes in cathepsins B-1 and D, neutral proteinase and 2',3'-cyclic nucleotide-3'-phosphohydrolase activities in monkey brain with experimental allergic encephalomyelitis |
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Authors: | K R Govindarajan H C Rauch J Clausen E R Einstein |
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Institution: | 1. Neurochemical Institute, 58, Rådmandsgade, Copenhagen Denmark;2. Department of Medical Microbiology, Stanford University School of Medicine, Stanford, Calif. U.S.A.;3. Institute of Hygiene, Preventive Medicine and Environmental Science, Odense Denmark;4. Department of Physiology and Institute of Human Development, University of California, Berkeley, Calif. U.S.A. |
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Abstract: | Myelin basic protein modified with 2′-hydroxy-5′-nitrobenzylbromide or homologous monkey white matter was used to induce experimental allergic encephalomyelitis (EAE) in rhesus monkeys. Altogether 7 monkeys with and without EAE were studied. Macroscopic lesions (plaques), normal-appearing white matter of normal rhesus monkey brains were removed and assayed for cathepsins B-1 and D, neutral proteinase and 2′-3′-cyclic nucleotide-3′-phosphohydrolase activities. Lymph nodes from 2 monkeys, 1 with and 1 without EAE were also included in the present investigation.Cathepsins B-1 and D and neutral proteinase were significantly increased in lesion areas of EAE monkeys when compared with the findings in normal brain material. The enzyme 2′,3′-cyclic nucleotide-3′-phosphohydrolase was found to be significantly decreased in the lesions when compared to the normal-appearing white matter areas of the same animals. The change parallels the reduction of other well-established constituents of the myelin, giving support to the concept that the enzyme is a component of the myelin.The results of the enzyme assays are discussed in relation to the pathogenesis of CNS lesions in EAE. |
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Keywords: | Requests for reprints should be addressed to: J Clausen The Neurochemical Institute Rådmandsgade 58 2200 Copenhagen N Denmark |
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