重组腺病毒介导CD自杀基因联合野生型p53基因对直肠癌细胞的杀伤作用

目的 观察重组腺病毒介导胞嘧啶氨酶（ＣＤ）基因和人野生型ｐ５３基因共转染对直肠癌细胞的杀伤作用。方法 用携带ＣＤ／ｐ５３、ＣＤ、ｐ５３基因腺病毒感染直肠癌细胞ＳＷ８３７，计数瘤细胞集落数，采用四唑蓝比色（ＭＴＴ法）检测瘤细胞存活率。于６０只裸鼠移植瘤内注射重组腺病毒、磷酸盐缓冲液（ＰＢＳ），测定肿瘤生长抑制率。结果 用ＣＤ／ｐ５３、ＣＤ、ｐ５３重组腺病毒感染ＳＷ８３７细胞，加５－ＦＣ后细胞集落形成分别为：６、３８、６９。裸鼠移植肿瘤生长抑制率分别为７８．６％、５６．５％、２２．３％。ＣＤ／ｐ５３重组腺病毒感染组肿瘤细胞集落形成和存活率下降最显著（Ｐ〈０．０１），对肿瘤的抑制作用明显。结论 ＣＤ自杀基因与野生型ｐ５３基因共转染对肿瘤细胞有更强的杀伤作用。

Antitumor effects of coinfection of CD suicide gene and wild-type p53 gene with adenovirus mediation on rectal cancer cells

Objective To investigate antitumor effects of cytosine deaminase (CD) suicide gene/5 fluorocytosine (5 FC) and human wild type p53 gene with adenovirus mediation on rectal cancer cells. Methods With adenovirus carrying CD/p53, CD, p53 genes, SW837 rectal cancer cells were infected, and plating efficiency was counted. Surval rate of the tumor cells was tested with MTT method. The recombinant adenovirus were injected into xenograft tumors of SW837 cells in 60 nude mice. Suppression rate of tumor growth was examined. Results Plating efficiency of the SW837 cells infected by recombinant adenovirus with CD/p53, CD, p53 genes, adding 5 FC in each group, was 6, 38, 69 respectively. Suppression rate of tumor growth was 78.6%, 56.5%, 22.3% in ilka group. The decreases of plating efficiency and surval rate of tumor cells in CD/p53 recombinant adenovirus infection group were the most significant ( P <0.01 ). And a strong suppression of xenograft tumor was observed by combination of CD/5 FC with wild type p53. Conclution Coinfection of CD and wild type p53 genes has more powerful antitumor actions.