| 分光光度法测定不同产地荷叶药材总黄酮的含量 |
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| 引用本文： | 曹娟,王福刚,陈静,王彩燕.分光光度法测定不同产地荷叶药材总黄酮的含量[J].泰山医学院学报,2010,31(5):358-360. |
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| 作者姓名： | 曹娟 王福刚 陈静 王彩燕 |
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| 作者单位： | 1. 泰山医学院药学院,山东,泰安,271016
2. 泰山医学院药学院2006级制药工程专业,山东,泰安,271016 |
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| 摘 要： | 目的比较不同产地荷叶药材总黄酮的含量。方法采用紫外-可见光分光光度法,以芦丁为对照品,NaNO3-Al（NO3）3-NaOH显色,在500 nm处测定总黄酮含量。结果在14.43~72.15μg.ml-1范围内,吸光度与浓度线性关系良好（r=0.9995）,平均回收率为100.64%（RSD=0.90%）,不同产地药材总黄酮含量范围为1.88%~10.99%。结论不同产地荷叶药材中总黄酮含量存在较大差异。
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| 关 键 词： | 荷叶 总黄酮 分光光度法 |
Determination of flavonoids in Folium Nelumbinis from different areas by spectrophotometry |
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| CAO Juan,WANG Fu-gang,CHEN Jing,WANG Cai-yang.Determination of flavonoids in Folium Nelumbinis from different areas by spectrophotometry[J].Journal of Taishan Medical College,2010,31(5):358-360. |
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| Authors: | CAO Juan WANG Fu-gang CHEN Jing WANG Cai-yang |
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| Institution: | 1.Dept.of Pharmacology,Taishan Medical University,Taian 271016,China;2.Undergraduate Student of 2006 of Pharmaceutical Engineering Subject,Dept.of Pharmacology,Taishan Medical University,Taian271016,China) |
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| Abstract: | Objective：To develop a method to determinate flavonoids in Folium Nelumbinis from different areas.Methods： The flavonoids in Folium Nelumbinis were determined by spectrophotometry at 500nm.Rutin was used for comparison.The color reagent was a mixture of NaNO2-Al（NO3）3-NaOH solution.Results： The calibration curve was linear in the range of 14.43~72.15 μg·ml-1（r=0.9995）.The average recovery was 100.64%（RSD=0.90%）.The contents of flavonoids from different areas are 1.88%~10.99%.Conclusion： The yields of the flavonoids from different areas are different.. |
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| Keywords: | folium Nelumbinis total flavonoids spectrophotometry |
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