Conductive conduit small gap tubulization for peripheral nerve repair |
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| Authors: | Xingxing Fang Jiuxu Deng Wei Zhang Haichang Guo Fei Yu Feng Rao Qicheng Li Peixun Zhang Shulin Bai Baoguo Jiang |
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| Institution: | Department of Orthopedics and Trauma, Peking University People''s Hospital, Beijing 100044 China.; Department of Materials Science and Engineering, CAPT/HEDPS, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Engineering, Peking University, Beijing 100871 China.; Department of Trauma Medicine Center, Peking University People''s Hospital, Beijing 100044 China ; National Center for Trauma Medicine, China |
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| Abstract: | Despite advances in surgical techniques, functional recovery following epineurial neurorrhaphy of transected peripheral nerves often remains quite unsatisfactory. Small gap tubulisation is a promising approach that has shown potential to traditional epineurial neurorrhaphy in the treatment of peripheral nerve injury. Thus, the goal of this study is to evaluate sciatic nerve regeneration after nerve transection, followed by small gap tubulization using a reduced graphene oxide-based conductive conduit. In vitro, the electrically conductive conduit could promote Schwann cell proliferation through PI3K/Akt signaling pathway activation. In vivo, the results of electrophysiological and walking track analysis suggest that the electrically conductive conduit could promote sensory and motor nerve regeneration and functional recovery, which is based on the mechanisms of selective regeneration and multiple-bud regeneration. These promising results illustrate electrically conductive conduit small gap tubulization as an alternative approach for transected peripheral nerve repair.rGO-based conductive nerve conduit as a scaffold to bridge peripheral nerve transected injury and 2 mm gap provides a suitable microenvironment for axons selective regeneration. |
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