达立通颗粒挥发油GC指纹图谱及GC-MS成分分析

目的 建立达立通颗粒挥发油GC指纹图谱，并对挥发油成分进行分析。方法 以Agilent HP-5（30m×0.32mm,0.25μm）石英毛细管柱进行分离，程序升温，氢火焰离子检测器（FID）检测。建立10批样品图谱共有模式，结合化学计量学法分析。运用GC-MS与NIST11.L标准谱库检索，鉴定挥发油成分，并推测各成分来源。结果 从10批样品共有图谱中选取13个共有峰，各成分相似度均大于0.998，样品可分为3类。通过GC-MS共鉴定出56种成分，包含11个共有峰。结论 建立的达立通颗粒挥发油GC指纹图谱，精密度、重复性与稳定性良好，可为达立通颗粒挥发油质量控制提供参考依据。

GC fingerprint and GC-MS composition analysis of volatile oil in Daliton granules

ABSTRACT: OBJECTIVE To establish the GC fingerprint of the volatile oil of Daliton granules and to analyze the volatile oil components. METHODS The separation was performed on an Agilent HP-5 (30m×0.32mm,0.25μm) quartz capillary column with programmed temperature rise and hydrogen flame ionization detector (FID) detection. A common pattern of 10 batches of samples was established and analyzed by a combination of chemometric methods. The volatile oil components were identified using GC-MS with NIST11.L standard library search, and the origin of each component was inferred. RESULTS Thirteen common peaks were selected from the common chromatograms of 10 batches of samples, and the similarity of each component was greater than 0.998, and the samples could be classified into three categories. A total of 56 components were identified by GC-MS, including 11 shared peaks. CONCLUSION The GC fingerprint of Dalitong granules volatile oil was established with good precision, reproducibility and stability, which can provide a reference basis for the quality control of Dalitong granules volatile oil.