丹参水提液对抗大鼠泼尼松性骨质疏松的作用研究

目的:观察中药丹参水提液对泼尼松所致的大鼠骨质疏松的对抗作用。方法:50只大鼠随机分成正常对照组、泼尼松组和丹参水提液高、中、低剂量组,每组10只。分别灌喂相应药物,每日1次,连续8周后取血清检测生化指标,对骨进行组织切片检查,长度、宽度以及骨质的测量。结果:泼尼松组大鼠股骨重量、骨羟脯氨酸和骨钙含量比正常对照组明显降低,骨髓腔中脂肪组织增多(P<0.01),血浆胆固醇含量上升、碱性磷酸酶和高密度脂蛋白含量下降(P<0.05)。丹参水提液能有效提高骨的重量和骨质的含量(P<0.05),减少骨髓腔中脂肪的含量,升高碱性磷酸酶和高密度脂蛋白含量(P<0.05),但量效关系不明显。结论:泼尼松可抑制大鼠的骨生长及引起骨丢失,丹参水提液对其有较好的对抗作用。

Effects of Salvia miltiorrhiza Bge water extract on prednisone -induced osteoporosis in rats

AIM :To investigate the effect of Salvia miltiorrhiza Bge water extract on osteoporosis which induced by prednisone in rats.METHODS :Fifty SD rats were randomly divided into control group,prednisone group and Salvia miltiorrhiza Bge high dose group,middle dose group and low dose group,with 10 rats per group.Rats in the control group were treated with dissolvant,the others were treated with prednisone firstly,and then,the rats in prednisone group were treated with dissolvant,the 3 groups were treated with high dose,middle dose or low dose water extract of Salvia miltiorrhiza Bge respectively,once a day.After 8 weeks,the bone histomorphometric analysis of thighbone were performed in undecalcified sections,the content of Ca 2 and hydroxyproline(Hyp)were measured,the length and width of the thigh bone were tested.RESULTS :The bone weight,bone Hyp and bone Ca 2 in prednisone group were lower than those in control group.The fat in the marrow,the cholesterin in plasma were increased but ALP and HDL were reduced(P < 0.05).Salvia miltiorrhiza Bge water extract could increase the bone weight,reduce the fat in the marrow and increase the ALP and HDL(P < 0.05),but the relationship between dose and efficacy was not significant.CONCLUSION :Prednisone can restrain bone growth and induce bone loss,while Salvia miltiorrhiza Bge water extract has good protective effect.