Contribution of immunofluorescence to identification and characterization of antineutrophil cytoplasmic antibodies in inflammatory bowel disease |
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Authors: | Dorra Bouzid Samy Haddouk Ali Amouri Youssef Ben Hadj Hmida Nabil Tahri Hatem Masmoudi |
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Institution: | (1) Immunology Department, Habib Bourguiba Hospital, 3029 Sfax, Tunisia |
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Abstract: | We evaluated the combined use of different fixatives for the identification of atypical perinuclear anti-neutrophil cytoplasmic
antibodies (p-ANCA) in patients with inflammatory bowel diseases (IBD) by indirect immunofluorescence (IIF). Sera from 59
ulcerative colitis (UC) and 37 Crohn’s disease (CD) patients, and from 64 healthy controls were studied. The IIF on ethanol-,
formalin-, and methanol-fixed neutrophils was used for the detection of ANCA. Enzyme linked immunosorbant assay (ELISA) was
performed to identify the antigens recognized by ANCA. ANCAs were present in 35 of 59 (59.3%) UC patients and in 10 of 37
(27.02%) CD patients. Atypical p-ANCA positivity was strongly associated with UC disease (44.1% in UC vs. 8.1% in CD; p = 0.0002). The combined application of different fixatives contributed to make easy the differentiation between typical p-ANCA
and atypical p-ANCA. Atypical p-ANCA determination appears to be a useful parameter for the distinction between UC and CD. |
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