Techniques for the preparation and analysis of a liposomal formulation of lisomustine |
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| Authors: | K. V. Kostin E. V. Ignat’eva E. V. Tazina V. M. Pechennikov N. A. Oborotova |
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| Affiliation: | (1) N. N. Blokhin Russian Oncological Scientific Center, Russian Academy of Medical Sciences, Moscow, Russia;(2) State Higher Professional Educational Institution I. M. Sechenov Moscow Medical University, Russian Ministry of Health and Social Development, Moscow, Russia; |
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| Abstract: | The optimal composition and production technique were identified for preparation of liposomal formulations of lisomustine. The stability of this formulation was increased by lyophilization of the liposomal dispersion using a cryoprotector, i.e., 10% sucrose solution. The lisomustine content in lysosomes was assayed by spectrophotometry at a wavelength of 230 nm. Uptake of lisomustine into freshly prepared liposomes amounted to 61.2 ± 0.4% and particle size was 170 ± 20 nm. |
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