青蒿素衍生物抗卡氏肺孢子虫体外作用的研究

目的 研究不同浓度青蒿素衍生物双氢青蒿素、青蒿琥酯对体外培养卡氏肺泡子虫的作用。方法 接种卡氏肺孢子虫（Pc）于人肝癌细胞系（HepG-2)。4h加入试验药物和对照试剂。双氢青蒿素药物浓度分别为：100、50、10、5、0．5μmol/L;青蒿琥酯药物浓度分别为：100、50、10、5μmol/L;对照药喷他脒：15μmol/L。以后每隔1d取培养液观察,分别作六亚甲基四胺银（GMS）和Diff-Quik(DQ)染色计数Pc包囊和滋养体数目。结果 Pc滋养体和包囊在体外培养细胞上的生长高峰出现于第5d,双氢青蒿素浓度为50μmol／L和100μmol／L时对Pc滋养 的抑制率分别为88．0％与90．1％,与喷他脒15μmol/L的抑制率90．0％相当;而青蒿琥酯浓度为100μmol/L时方可达到Pc滋养体抑制率83．4％。Pc包囊抑制率,双氢青蒿素和青蒿琥酯浓度为100μmol/L分别为67．5％和67．3％,与喷他脒的抑制率75．0％之间无统计学差异。结论 双氢青蒿素和青蒿琥酯在一定浓度时67．3％,对Pc的抑制作用与喷他脒相当;而双氢青蒿素的抑制作用略高于青蒿琥酯。两者对Pc滋养体的抑制率均高于对Pc包囊。

In vitro study of the anti-pneumocystis carinii effect of arteminsin derivatives

Objective　In vitro study was carried out to assess the effect of dihydroarteminsinin(DHA) and sodium artesunate(ATS) on pneumocystis carinii(Pc). Methods　The drugs were added into HepG-2 cell cultures containing Pc cysts. The concentrations of dihydroarteminsinin (DHA) were 100, 50, 10, 5, 0.5 μmol/L respectively. The concentrations of sodium artesunate (ATS) were 100, 50, 10, 5 μmol/L respectively and pentamidine (PTD) was 15 μmol/L as a control. The supernatants were withdrawn every other day and evaluated for Pc trophozoite and cyst counts by GMS and DQ stain. Results　The peak of Pc multiplication was observed on 5th day. The inhibition rates of Pc trophozoites by DHA 50 μmol/L, 100 μmol/L and ATS 100 μmol/L were quite similar to that of pentamidine (PTD) 15 μmol/L. Conclusion　DHA and ATS can inhibit the growth of Pc as same as PTD, and DHA was slightly better than ATS.