| 白介素-6对体外培养的黑素细胞影响的实验研究 |
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| 引用本文： | 林新瑜,段西凌,林鸿刚,何飞. 白介素-6对体外培养的黑素细胞影响的实验研究[J]. 四川医学, 2011, 32(3): 317-319 |
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| 作者姓名： | 林新瑜 段西凌 林鸿刚 何飞 |
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| 作者单位： | 1. 四川省人民医院皮肤病性病研究所,人类疾病基因组学实验室,四川,成都,610072
2. 四川大学国家生物治疗重点实验室,四川,成都,610041 |
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| 摘 要： | 目的建立正常人表皮黑素细胞体外培养体系,研究白介素-6（Interleukin-6I,L-6）对体外培养的黑素细胞的影响。方法采用四甲基偶氮唑盐（MTT）法测定白介素-6对黑素细胞活力的影响,NaOH裂解法测定黑素合成,流式细胞仪检测黑素细胞的凋亡率。结果白介素-6对黑素细胞活力有抑制作用,能使细胞增殖力降低,黑素合成减少,增加黑素细胞的凋亡率。结论白介素-6对体外培养的黑素细胞增殖及黑素生成都有抑制作用,这为临床应用白介素-6治疗色素性疾病提供了实验依据。
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| 关 键 词： | 白介素-6 黑素细胞 |
Effects of interleukin-6 on cultured human melanocytes in vitro |
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| Affiliation: | LIN Xin-yu,DUAN Xi-ling,LIN Hong-gang,et al.(1.Department of Dermatology,Laboratory of Human Disease′s Genetics,The People′s Hospital of Sichuan,Chengdu,Sichuan 610072;2.State Key Laboratory of Biotherapy,Sichuan University,Chengdu,Sichuan 610041,China) |
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| Abstract: | Objective Establishing the culture of human normal melanocytes in vitro,and studying effects of Interleukin-6 on cultured human melanocytes.Methods MTT method,NaOH2 assay were employed to measure the cell viability,melanin synthesis of melanocytes,apoptosis rates of melanocytes were detected by using flow cytometer.Results Interleukin-6 induced a mild effect on melanocytic viability,while it showed a significant decrease in the cell counts,melanin synthesis and cell apoptosis.Conclusion Interleukin-6 can in... |
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| Keywords: | interleukin-6 melanocytes |
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