逆灸命门穴对正常大鼠力竭游泳时间及HPA轴的影响

目的:观察逆灸命门穴对大鼠力竭游泳时间及游泳力竭后下丘脑—垂体—肾上腺(HPA)轴的影响。方法:将大鼠按游泳能力随机分为空白对照组、逆灸命门组、力竭对照组、逆灸命门+力竭组,分别干预20d后进行力竭游泳,观察力竭时间的差别,并采用生化法检测下丘脑促肾上腺皮质激素释放激素(CRH)、垂体促肾上腺皮质激素(ACTH)、肾上腺皮质醇(CORT)的含量。结果:逆灸命门+力竭组力竭游泳时间较力竭对照组明显延长(P0.05)。各组内HPA轴的3种激素表现同步:与空白对照组相比,逆灸命门组大鼠的下丘脑CRH、垂体ACTH、肾上腺CORT含量无明显变化;力竭对照组大鼠的下丘脑CRH、垂体ACTH、肾上腺CORT含量极显著升高(P0.01);与力竭对照组比较,逆灸命门+力竭组大鼠的下丘脑CRH、垂体ACTH、肾上腺CORT含量明显降低(P0.01)。结论:运动力竭后HPA轴出现过度应激,逆灸命门穴可缓和其应激程度,这可能是逆灸命门穴能提高大鼠的运动耐力,延长其游泳力竭时间的部分机制。

Effect of preventive moxibustion at Mingmen(DU-4) on swimming exhausted time and HPA axis hormone of rats

Objective: To observe the effect of preventive acupuncture at Mingmen(DU-4) on exercise tolerance and HPA axis hormone of rats.Methods: Randomly divided rats into normal control group,Mingmen moxibustion group,exhaustion control group,Mingmen moxibustion + exhaustion group according to their swimming exhausted time.Rats received a exhaustive swimming after 20 days of intervention.Exhausted time of swimming rats were recorded.The hypothalamus CRH,pituitary ACTH,adrenal gland CORT of rats were detected by biochemical method and compared.Results: The swimming exhausted time of Mingmen moxibustion + exhaustion group were significantly increased compare to exhausted control group(P<0.05).Three types of hormone of HPA axis in each group have the same trend: Compared with normal control group,the hypothalamus CRH,pituitary ACTH,adrenal gland CORT of Mingmen moxibustion group had no significant differences.The HPA axis hormone of exhausted control group was significantly increased(P<0.01).The HPA axis hormone of Mingmen moxibustion + exhaustion group were significantly lower than that of the exhausted control group(P<0.01).Conclusions: Preventive moxibustion at Mingmen(DU-4) could improve the exercise tolerance of rats and prolong their swimming exhausted time.Its physiological mechanism might be related to easing excessive stress of HPA axis on exhaustive exercise.