206例抗-HCV抗体检测阳性分析 |
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引用本文: | 江跃红,迟伟群,姚玉虹,张超民,隋晓凤. 206例抗-HCV抗体检测阳性分析[J]. 黑龙江医学, 2010, 34(3): 211-212. DOI: 10.3969/j.issn.1004-5775.2010.03.022 |
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作者姓名: | 江跃红 迟伟群 姚玉虹 张超民 隋晓凤 |
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作者单位: | 哈尔滨医科大学附属第四医院,黑龙江,哈尔滨,150001;哈尔滨医科大学附属第四医院,黑龙江,哈尔滨,150001;哈尔滨医科大学附属第四医院,黑龙江,哈尔滨,150001;哈尔滨医科大学附属第四医院,黑龙江,哈尔滨,150001;哈尔滨医科大学附属第四医院,黑龙江,哈尔滨,150001 |
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摘 要: | 目的分析丙型肝炎病毒(HCV)抗体酶联免疫吸附试验(ELISA)阳性吸光度值/临界值(S/CO)与HCVRNA定量的相关性。方法采用实时荧光定量PCR检测HCV-RNA及ELISA法检测抗-HCV。结果206份抗-HCV阳性样本中,HCV-RNA阴性33例,将HCV-PCR阳性173例按S/CO值分组。1.0≤S/CO<2.0者22例,占12.8%;2.0≤S/CO<3.8者35例,占20.2%;S/CO≥3.8者116例,占67%。经统计学分析,两者有明显的相关性。结论ELISA法检测抗-HCV在S/CO≥3.8时,才高度预示抗-HCV呈阳性状态。本文作者建议对S/CO≥3.8的标本做确认实验。
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关 键 词: | 抗-HCV HCV-RNA 实时荧光定量PCR 丙型肝炎病毒 |
206 Cases Anti-HCV Antibody Positive Detection Result Analysis |
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Affiliation: | JIANG Yue-hong,CHI Wei-qun,YAO Yu-hong,et al.(The Fouth Affiliated Hospital of Harbin Medical University,Harbin 150001,CHINA) |
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Abstract: | Objective To study the relativity between s/co numerical value of anti-HCV antibody positive detectiom and HCV-RNA quantitation. Methods Applied ELISA and HCV-PCR technology test anti-HCV and HCV-RNA. Results In the samples of 206 cases anti-HCV antibody positive detectiom ,33 cases were negative result of HCV-RNA,the detection result was grouped by S/CO ratios.22 cases 1.0≤S/CO2.0,the percent of 12.8%;35 cases 2.0≤S/CO3.8,the percent of 20.2%;116 cases S/CO≥3.8,the percent of 67%. Conclusion While the S/CO≥3.8 the anti-HCV would be positive by the measure method of ELISA and we suggest that we should do the affirmance experiment at the same time. Obviously the two cases were relative. |
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Keywords: | HCV-RNA |
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