| HPLC法测定感冒止咳颗粒中葛根素的含量 |
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| 引用本文： | 刘海霞,杨志伟,邹文斌.HPLC法测定感冒止咳颗粒中葛根素的含量[J].亚太传统医药,2013,9(5):52-54. |
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| 作者姓名： | 刘海霞 杨志伟 邹文斌 |
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| 作者单位： | 刘海霞 (松原市药品检验所,吉林,松原,138000)；杨志伟 (松原市药品检验所,吉林,松原,138000)；邹文斌 (松原市药品检验所,吉林,松原,138000)； |
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| 摘 要： | 目的：建立以高效液相色谱法测定感冒止咳颗粒中葛根素含量的方法。方法：ZORBAX SB—C18（250mm×4．6mm，5μm），流动相为甲醇-水（28：72），流速为1．0mL／min，检测波长为250nm，柱温为30℃。结果：葛根素在0．0801～1．6024μg／mL范围内呈良好的线性关系。结论：本方法简便、准确、分离效果好、线性范围宽、灵敏度高，可用于本品的质量控制。
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| 关 键 词： | 高效液相色谱法 感冒止咳颗粒 葛根素 含量 |
HPLC in Determination of Cold Zhike Granules of Puerarin Content |
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| Liu Haixia,Yang Zhiwei,Zou Wenbin.HPLC in Determination of Cold Zhike Granules of Puerarin Content[J].Asia-Pacific Traditional Medicine,2013,9(5):52-54. |
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| Authors: | Liu Haixia Yang Zhiwei Zou Wenbin |
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| Institution: | (Drug inspection institutes of SongYuan,Jilin 138000,China) |
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| Abstract: | Objective：To establish a high performance liquid chromatography determination of puerarin in cold cough particle con tent of method. Methods：ZORBAX SB-C18 （250mm×4.6mm,5μm）, mobile phase was methanol and water （28 ：72）, flow rate is 1.0mL/min, detection wavelength was 250nm, column temperature was 30℃. Results： Puerarin of 0. 080 1 - 1. 602 4μg/mL .showed good linear relationship. Conclusion：This method is simple and accurate, good separating effect, wide linear range, high sensitivity, and can be used for the quality control of the product. |
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| Keywords: | High Performance Liquid Chromatography Cold Zhike Granules Puerarin Content |
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