| HPLC测定倒卵叶五加冲剂中紫丁香苷含量研究 |
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| 引用本文： | 曹爱兰,张俏,史文静,杜文娟.HPLC测定倒卵叶五加冲剂中紫丁香苷含量研究[J].亚太传统医药,2013,9(5):33-34. |
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| 作者姓名： | 曹爱兰 张俏 史文静 杜文娟 |
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| 作者单位： | 曹爱兰 (陕西中药研究所,陕西,咸阳,712000)； 张俏 (陕西中药研究所,陕西,咸阳,712000)； 史文静 (陕西中药研究所,陕西,咸阳,712000)； 杜文娟 (陕西中药研究所,陕西,咸阳,712000)； |
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| 摘 要： | 目的:建立测定倒卵叶五加冲剂中紫丁香苷含量的HPLC测定方法。方法:色谱柱:kromasil C18柱(150mm×4.6mm,5μm);流动相:乙腈-水(10∶90);流速:1mL.min-1;检测波长:265nm;柱温:35℃。结果:经检测,紫丁香苷的线性范围为0.0192～0.1536mg/mL,Y=9.846+2.824X×104 C(r=0.999 6),加样回收率为97.7%。结论:HPLC测定倒卵叶五加冲剂中紫丁香苷含量,方法稳定可靠,操作简便,可作为五加参冲剂质量控制指标。
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| 关 键 词： | 五加参冲剂 紫丁香苷 HPLC 含量测定 |
Determination of Syringin in Wujiashen GranuLes by HPLC |
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| Cao Ailan,Zhang Qiao,Shi Wenjing,Du Wenjuan.Determination of Syringin in Wujiashen GranuLes by HPLC[J].Asia-Pacific Traditional Medicine,2013,9(5):33-34. |
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| Authors: | Cao Ailan Zhang Qiao Shi Wenjing Du Wenjuan |
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| Institution: | (Shanxi Institute of Traditional Chinese Medicine Shanxi 712000,China) |
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| Abstract: | Objective：To establish determination method on content of syringin in Daoluanyewujia granuLes by HPLC. Methods： The analysis was carried out with a kromasil C18 （150mm×4.6mm,5μm） column, and acetonitrile-water （10：90） as mobile phase, with a flow rate of 1.0 mL ; min-1 ,the detection wavelength was 265 nm and the column temperature was 35 ;C. Results：There is a wonderful linerarity in the range of 0.0192--0. 153 6 rag, the regression equation is follows： Y= 9. 846 ＋ 2. 824 × 10^4C, r; 0. 9996;the average recovery rate is 97.7 ;. Conclusion：The content determination method is sensitive, stable and reliable and also with good reproducible. The method could control the quality of Daoluanyewujia granuLes. |
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| Keywords: | Daoluanyewujia GranuLes Syringin HPLC Content Determination |
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