G蛋白偶联受体120在肥胖患者腹部皮下和大网膜脂肪组织中的表达及其影响因素

目的探讨游离脂肪酸功能受体G蛋白偶联受体120（GPRl20）在肥胖患者腹部皮下脂肪组织和大网膜脂肪组织中mRNA表达情况及其相关的影响因素。方法于2010年9月至2011年8月选取广州医学院第三附属医院施行慢性胆囊炎择期手术的非超重患者对照组，体质指数（BMI）〈23kg／m。]39例和肥胖患者（肥胖组，BMI≥25kg／m2）40例，测量体重、腰臀围、血糖、血脂等指标，用逆转录．聚合酶链反应法（RT．PCR）检测腹部大网膜与皮下脂肪组织GPRl20mRNA的表达水平。采用t检验、Pearson相关分析、偏相关分析和多元逐步回归进行统计学分析。结果（1）大网膜组织中的GPRl20mRNA表达在肥胖组（0．876±0．076）和对照组（0．621±0．052）均高于两组皮下脂肪组织中的水平（0．479±0．028、0．421±0．035），差异有统计学意义（t值分别为-2．546、-2．211，均P〈0．05）。肥胖组大网膜的GPRl20mRNA表达量（0．876±0．076）高于对照组（0．621±0．052），差异有统计学意义（t=-2．348，P〈0．05），皮下脂肪GPRl20mRNA表达量在肥胖组（0．4794-0．028）与对照组（0．4214-0．035）之间差异无统计学意义（t=-0．584，P〉0．05）。（2）以大网膜脂肪组织GPRl20mRNA表达量为应变量，仅稳态模型胰岛素抵抗指数和腰臀比进入分析模型，所得模型R。=0．3730，标准偏回归系数分别为0．227和0．321。皮下脂肪GPRl20mRNA表达量与年龄、血酯等其他因素均无相关性（均P〉0．05）。结论肥胖患者腹部大网膜脂肪组织GPRl20mRNA表达明显升高，腹型肥胖、胰岛素抵抗是大网膜脂肪组织GPRl20表达水平的主要影响因素。

Expression of G-protein coupled receptor 120 mRNA and related factors in subcutaneous and omental adipose tissues of obese subjects

Objective To examine the expression of G-protein coupled receptor 120 （GPR120） mRNA in abdominal subcutaneous and omental adipose tissues and related factors in obese subjects. Methods From September 2010 to August 2011, 39 non overweight subjects （body mass index （BMI） 〈 23 kg/m2 ） and 40 obese patients （ BMI 〉 25 kg/m2 ） who underwent eholeeystectomy in the Third Affiliated Hospital of Guangzhou Medical College were enrolled in this study. Body weight, waist-hip ratio （WHR） , blood glucose, and lipids,etc, were measured. The expression of GPR120 mRNA in abdominal subcutaneous and omental adipose tissues were measured by RT-PCR. The t test, Pearson correlation analysis, partial correlation analysis and multi-stepwise regression were used for data analysis. Results （1） In obese and control group, the GPR120 mRNA expression in omental adipose tissue （0. 876 ±0. 076, O. 621 ± 0. 052） were significantly higher than those in subcutaneous adipose tissue （0. 479 ± 0. 028, 0. 421 ±0. 035 ,t = -2. 546 or -2. 211, respectively,both P 〈0. 05）. The GPR120 mRNA expression in omental adipose tissue （ 0. 876 ± 0. 076 ） was significantly higher in obese group than that in control group （0. 621 ± 0. 052, t = - 2. 548, P 〈 0. 05） . The differences in the expression of GPR120 mRNA in abdominal subcutaneous adipose tissue between the two groups were not significant （0. 479 ±0. 028 vs 0. 421±0. 035, t = -0. 584, P 〉 0. 05 ）. （2） Multi-stepwise regression analysis demonstrated that the expression of GPR120 mRNA in omental adipose tissue was significantly correlated with HOMA-IR and WHR （R2 = 0. 3730, standard partial regression coefficient were 0. 227 and 0. 321, respectively）. The expression of GPR120 mRNA in abdominal subcutaneous adipose tissue was not correlated with other factors. Conclusion The GPR120 mRNA expression in abdominal omental adipose tissue was significantly higher in obese subjects than that in control group. Abdominal obesity and HOMA-IR were the main independent factors influencing the expression of GPR120 mRNA in omental adipose tissue.