乙型肝炎病毒基因型与病毒复制的关系

检测慢性乙型肝炎患者血清乙型肝炎病毒(HBV)基因型和病毒载量(HBV-DNA定量),探讨它们之间的关系。用微板核酸杂交-ELISA方法对HBV进行基因分型,用PCR荧光定量检测血清HBV-DNA水平,共318例。检测到HBV B型111例(35％);C型128(40％);混合型(B+C,C+D,B+C+D)45例(14％);D型2例;F型2例;未分型30例(9.4％);没有发现A、E型。结果发现,C型和混合型HBV的血清DNA水平高于B型(1.14×107vs2.2×107vsl.60×106拷贝/ml,P<0.05);而混合型HBV的血清DNA水平与C型无差别(P=0.127)。研究提示,我国HBV以B,C两基因型为主,HBV基因型可能是影响HBV复制的重要因素之一。

The relationship between viral genotype and viral replication in patients with chronic hepatitis B

To identify the relationship between viral genotype and replication of hepadtis B virus (HBV) in patients with chronic hepatitis B. The viral genotypes were determined by micro - board nucleic acid hybridization - ELISA, and the HBV - DNA levels were detected by quantitative PCR based on fluorescence assay. Genotype B was detected in 111 cases (35%); genotype C was detected in 128 cases (40%); mixed genotypes of B+C or C + D or B+C+D in 45 cases (14%); genotype D and F was detected in 2 cases, respectively; 30(9.4% ) cases were ungenotyped. None of them bad genotyped A or E. The HBV - DNA levels in genotype C and mixed genotype were significantly higher than those with genotype B, while the difference of the DNA levels between genotype C and mixed genotype was not found. These results suggest that the predominant genotypes of HBV in patients with chronic hepatitis B in China are B and C, and the genotype may be an important determinant of viral replication in patients with chronic hepatitis B.