消痰解郁方对慢性应激荷瘤大鼠行为的影响及其机制

目的：观察慢性应激对荷瘤大鼠行为的影响，研究抗抑郁方剂消痰解郁方的作用及其机制。 方法：60只Wistar大鼠随机分为正常组、荷瘤组、应激组、应激荷瘤组、消痰解郁方组和氟西汀胶囊组。经过不同的处置后，观察各组大鼠糖水耗食量、行为学得分和体质量，酶联免疫吸附测定法检测血清肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）、白细胞介素6（interleukin-6，IL-6）含量，蛋白印迹法检测海马Bcl-2蛋白和磷酸化细胞外信号调节蛋白激酶（phosphorylated extracellular signal-regulated kinase 1/2，p-ERK1/2）蛋白表达水平，聚合酶链式反应检测海马组织Bcl-2 mRNA表达。 结果：慢性应激组及荷瘤组大鼠的糖水耗食量、行为学得分、体质量较正常组下降。荷瘤组及慢性应激组大鼠血清TNF-α、IL-6含量升高，海马组织Bcl-2蛋白、mRNA及p-ERK1/2蛋白水平均下降，其中荷瘤大鼠接受应激后较正常大鼠接受应激后血清TNF-α水平增高，同时海马Bcl-2 mRNA水平下降最为显著，差异有统计学意义（P〈0.05）。消痰解郁方能改善慢性应激大鼠及荷瘤大鼠的行为学指标，下调血清TNF-α、IL-6含量，提高Bcl-2、p-ERK1/2蛋白表达水平。 结论：荷瘤大鼠较正常大鼠在接受慢性应激后更易出现抑郁行为，消痰解郁方能改善荷瘤大鼠慢性应激后的行为学改变，其作用机制可能与调节荷瘤大鼠血清TNF-α含量，上调海马Bcl-2蛋白、p-ERK1/2蛋白的表达有关。

Effects of Xiaotan Jieyu Recipe on behaviors of tumor-bearing rats with chronic mild stress and the mechanism

Objective： To investigate the effects of unpredictable chronic mild stress and Xiaotan Jieyu Recipe （XTJYR）, a compound traditional Chinese herbal medicine, on the behaviors of Walker 256 tumor-bearing rats and to explore the mechanism.
Methods： Sixty Wistar rats were randomly divided into control group, stress group, tumor-bearing group, stress plus tumor-bearing group, fluoxetine group and XTJYR group. After treatment, sucrose solution consumption, score of ethology, body weight and serum levels of tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） were detected, expressions of Bcl-2 and phosphorylated extracellular signal-regulated kinase 1/2 （p-ERK1/2） proteins in hippocampus were detected by Western blotting method, and expression of Bcl-2 mRNA was measured by polymerase chain reaction method.
Results： Sucrose solution consumption, behavioral scores and body weight of rats were decreased in the stress group and the tumor-bearing group as compared with the control group. There were significant differences in expressions of Bcl-2 mRNA and Bcl-2 and p-ERK1/2 proteins in hippocampus and contents of serum TNF-α and IL-6 in the stress group and the tumor-bearing group as compared with those in the control group. XTJYR had the efficacy in improving behavioral scores of stress rats and tumor-bearing rats, down-regulating the contents of serum TNF-α and IL-6 and increasing the expressions of proteins of Bcl-2 and p-ERK1/2.
Conclusion： Tumor-bearing rats are prone to behave depressively after exposure to chronic mild stress and XTJYR can improve the behavioral scores of the rats. The mechanisms of XTJYR may relate to regulating contents of serum TNF-α and increasing the protein expressions of Bcl-2 and p-ERK1/2.