HIV/AIDS患者CD4~+ T细胞中CD25和CD8~+ T细胞中CD28的表达

目的探讨外周血CD4+ T细胞中CD25表达率和CD8+ T细胞中CD28表达率在HIV/AIDS患者发病中的作用。方法应用流式细胞仪荧光染色技术检测35例HIV/AIDS患者CD4+ T细胞中CD25表达和CD8+ T细胞中CD28的表达,以41名健康体检人员做对照。结果HIV/AIDS患者和健康对照组之间CD4+ T细胞中CD25表达率(27.51±4.23)%,(44.41±9.17)%,CD4+25+ T细胞占淋巴细胞的比例(2.00±1.42)%,(16.62±4.60)%,CD4+25- T细胞占淋巴细胞的比例(5.16±3.37)%,(21.03±6.19)%,CD8+ T细胞中CD28中的表达率(25.12±6.33)%,(44.24±8.61)%,CD8+28- T细胞占淋巴细胞的比例(36.85±8.98)%,(13.33±4.58)%的差异均有显著性(P<0.01),CD8+ 28+ T细胞占淋巴细胞的比例(12.31±4.14)%,(10.51±3.71)%差异无显著性(P>0.05)。结论HIV/AIDS患者CD25在CD4+ T细胞与CD28在CD8+ T细胞的表达率降低可能与HIV感染后引起的免疫缺陷有关,CD8+ CD28- T细胞的增加有助于促进HIV/AIDS患者的炎性反应和免疫激活。

Expressions of CD25 in CD4+T Cells and CD28 in CD8+T Cells in HIV/AIDS Patients

Objective To study the expressions of CD25 in CD4+ T cells and CD28 in CD8+ T cells in the peripheral blood of HIV/AIDS. Methods CD25 in CD4+ T cells and CD28 in CD8+ T cells in the peripheral blood of 35 HIV/AIDS and 41 normal control were detected by flow cytometr. Results It shows that in patients with HIV/AIDS and the normal subjects,the expression percent of CD25 in CD4+T cells(27.51±4.23)%,(44.41±9.17)%、CD4+25+T cells(2.00±1.42)%,(16.62±4.60)%、CD4+25-T cells(5.16±3.37)%,(21.03±6.19)%、CD28 in CD8+T cells(25.12±6.33)%,(44.24±8.61)%, CD8+28-T cells(36.85±8.98)%,(13.33±4.58)%had significantly different (P<0.01);CD8+28+T cells(12.31±4.14)%,(10.51±3.71)%had no significantly different (P>0.05). Conclusions The low expression of CD25 in CD4+ T cells and CD28 in CD8+ T cells in HIV infection patients was closely associated with Immunodeficiency by HIV infection, the increase of CD8+28-T cells help to promote HIV/AIDS patients with inflammatory response and immune activation.