HBV标志物定量与病毒载量关系探讨

目的 研究乙型肝炎病毒各血清标志物含量与HBV-DNA含量的关系.方法 采用时间分辨免疫荧光分析法(TRIFA)和实时荧光定量聚合酶链反应(FQ-PCR)分别测定1 486例乙肝患者血清中乙型肝炎病毒抗原抗体定量和病毒含量.结果 不同HBV标志物阳性模式的HBsAg定量存在显著差异,相同模式的HBsAg定量也差异极大.HBeAg( )模式的HBV-DNA显著高于HBeAb( )模式及HBeAg(-)HBeAb(-)模式(P＜0.01),而后两者间无显著差异(P＞0.05).HBsAg、HBeAg含量与HBV DNA正相关(r值分别为0.383、0.635,P=0.01),HBeAb含量与HBV DNA负相关(r=-0.563,P=0.01).HBsAg定量与HBeAg定量正相关(r=0.466,P=0.01),与HBeAb定量负相关(r=-0.524,P=0.01).结论 HBV标志物定量之间及与HBV DNA含量间存在一定相关性,但个体差异极大.全面了解HBV标志物含量及HBV DNA定量可更准确判断病毒复制、状态,为临床决策提供可靠依据.

Correlation of virus load to contents of serologic markers in HBV patients

Objective To explore the relationship between HBV load and the contents of serologic markers.Methods Time-resolved immunofluorometric assay(TRIFA) was used to detect the contents of HBV immunological markers and real-time fluorescent quantitative PCR(FQ-PCR) was used to measure the HBV DNA of 1 486 clinical serum samples.The data was analyzed by SPSS11.0.Results The HBsAg contents were extremely different in the same or different models of serologic markers.The HBV copies of HBeAg( ) patients were significantly more than HBeAb( ) and HBeAg(-)HBeAb(-) patients(P<0.01),but without statistical significance between HBeAb( ) and HBeAg(-)HBeAb(-) patients(P>0.05).The contents of both HBsAg and HBeAg were positively correlated with the load of HBV DNA(r=0.383,0.635,P=0.01),while HBeAb negatively correlated to it(r=-0.563,P=0.01).Direct correlation existed between the contents of HBsAg and HbeAg(r=0.466,P=0.01) while inverse correlation was observed between the contents of HBsAg and HbeAb(r=-0.524,P=0.01).Conclusion A certain correlation exists between the contents of serologic markers and DNA of hepatitis B but the great variance exists among individuals.Combining FQ-PCR with TRIFA method is profitable for judging the state of HBV viral replication roundly and provides scientific and reliable evidences for clinical decisions.