慢性乙型肝炎患者肝组织乙型肝炎病毒共价闭合环状DNA的定量检测

目的 探讨肝组织乙型肝炎病毒共价闭合环状DNA(HBV CccDNA)水平与病毒复制、肝组织损伤的关系.方法 应用荧光定量聚合酶链反应(PCR)方法检测46例慢性乙型肝炎患者肝组织HBV CccDNA水平,同时检测肝组织和血清中HBV DNA、肝细胞乙型肝炎表面抗原(HBsAg)和乙型肝炎核心抗原(HBcAg)表达及肝功能.结果 将肝组织中HBVCccDNA定量水平分为低(＜104copies/mg)、中(104～106copies/mg)、高(＞106 copies/mg)3组,3组中HBsAg阳性表达差异无统计学意义,但105～106copies/mg和＞107copies/mg组HBcAg阳性表达高于＜104 copies/mg组,分别为105～106copies/mg组HBcAg表达14例(63.6%)和＞107copies/mg组7例(77.8%)vs＜104copies/mg组2例(13.3%)(P＜0.01);HBeAg阳性组肝组织中HBV CccDNA和肝组织总HBV DNA均显著高于HBeAg阴性组,分别为肝组织中HBV CccDNAHBeAg阳性组(8.72±1.94)log10 copies/mg vs HBeAg阴性组(6.54±1.56)log10 copies/mg;肝组织中HBV DNAHBeAg阳性组(7.45±1.82)1og10 copies/mg vs HBeAg阴性组(5.27±1.48)log10 copies/mg(均P＜0.01);肝组织中HBV CccDNA与肝组织总HBV DNA、血清HBV DNA呈正相关(均P＜0.05);而与肝组织炎症程度、肝纤维化程度、丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)无相关性.结论 肝组织中HBV CccDNA定量能准确反映病毒复制水平,但不能将其视为肝组织损伤的标志.

Quantification of liver hepatitis B virus covalently closed circular DNA in patients with chronic hepatitia B

Objective To explore the relationship between liver hepatitis B virus covalently closed circular DNA(cccDNA) with HBV replication and liver injury.Methods The levels of liver HBV CccDNA,liver HBV DNA and serum HBV DNA in patients with chronic hepatitis B were detected by fluorescent quantitative polymerase chain reaction.The expression of HBsAg and HBcAg in liver tissue and liver function were also examined at the same time.Results The levels of liver HBV CccDNA were divided into low group(<104 copies/mg),m...